Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category

Anti-EBP50/NHERF-1 antibody [EBP-10] (ab9526)

Anti-EBP50/NHERF-1 antibody [EBP-10] (ab9526)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [EBP-10] to EBP50/NHERF-1
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human
  • Isotype: IgG2b

You may also be interested in

Product image
Anti-Myc tag antibody [9E11] - ChIP Grade (ab56)
Product image
Anti-RCC2 antibody (ab229153)
Product image
Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody (ab150518)
Product image
Anti-PRPF4 antibody [EPR17206(B)] - BSA and Azide free (ab251351)

Overview

  • Product name

    Anti-EBP50/NHERF-1 antibody [EBP-10]
    See all EBP50/NHERF-1 primary antibodies
  • Description

    Mouse monoclonal [EBP-10] to EBP50/NHERF-1
  • Host species

    Mouse
  • Specificity

    Human ERM (ezrin/radixin/moesin)-binding phosphoprotein of 50 kDa (EBP50) / Na+/H+ exchange regulatory factor (NHERF-1)
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human EBP50/NHERF-1.

  • Positive control

    • RAJI human lymphoma cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    EBP-10
  • Isotype

    IgG2b
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Epi / Endo-thelial
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Signal Transduction
    • Adapters
    • Transmembrane
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other

Images

  • Western blot - Anti-EBP50/NHERF-1 antibody [EBP-10] (ab9526)
    Western blot - Anti-EBP50/NHERF-1 antibody [EBP-10] (ab9526)
    All lanes : Anti-EBP50/NHERF-1 antibody [EBP-10] (ab9526) at 2 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size: 48 kDa
    why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-EBP50/NHERF-1 antibody [EBP-10] (ab9526)
    Flow Cytometry - Anti-EBP50/NHERF-1 antibody [EBP-10] (ab9526)
    Overlay histogram showing HepG2 cells stained with ab9526 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9526, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-EBP50/NHERF-1 antibody [EBP-10] (ab9526)

  •  
  • Product image

    Anti-EBP50/NHERF-1 antibody [EPR5562] (ab109430)

    Applications: IHC-P, WB

  •  
  • Product image

    Anti-EBP50/NHERF-1 antibody (ab225904)

    Applications: IHC-P, IP, WB

  •  
  • Product image

    Anti-EBP50/NHERF-1 antibody (ab88238)

    Applications: WB

  •  
  • Product image

    Anti-EBP50/NHERF-1 antibody (ab3452)

    Applications: IHC-P, WB

Clear all

Recently viewed products

  •  
  • Product image

    Anti-Histone H3 (di methyl K79) antibody [EPR17467] - ChIP Grade (ab177184)

  •  
  • Product image

    Anti-FBXO2 antibody [EPR7328(2)] (ab133717)

  •  
  • Product image

    Anti-DIAPH1 antibody [EPR7948] - BSA and Azide free (ab248325)

  •  
  • Product image

    Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (ab251115)

  •  
  • Product image

    Anti-Chromogranin C/SGII antibody [SGII 6B1/3] (ab20245)

  •  
  • Product image

    Anti-GKN2 antibody [EPR15377(B)] - BSA and Azide free (ab232624)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.