Anti-Histone H2B antibody - C-terminal (ab231899)
Key features and details
- Rabbit polyclonal to Histone H2B - C-terminal
- Suitable for: ChIP, Dot blot
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H2B antibody - C-terminal
See all Histone H2B primary antibodies -
Description
Rabbit polyclonal to Histone H2B - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ChIP HumanDot Recombinant fragment
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Immunogen
Synthetic peptide corresponding to Human Histone H2B (C terminal) conjugated to keyhole limpet haemocyanin. Containing an unmodified sequence from the C-terminal part of the protein.
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Positive control
- ChIP: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.05% Sodium azide, 0.05% Proclin 300
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
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ChIP - Anti-Histone H2B antibody - C-terminal (ab231899)
ChIP assays were performed using HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, ab231899 and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 10,000 cells using the SX-8G IP-Star automated system. A titration of the antibody consisting of 1, 2, 5, and 10 μg per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH promoter and for the inactive MYOD gene. Image shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
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Dot Blot - Anti-Histone H2B antibody - C-terminal (ab231899)A Dot Blot analysis was performed to test the cross reactivity of ab231899 with the peptide used for immunization of the rabbit and other peptides containing unmodifed sequences of different histones.
One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000.
