Anti-Kappa light chain antibody [RM103] (ab190484)
![Anti-Kappa light chain antibody [RM103] (ab190484)](https://www.abcam.com/ps/products/190/ab190484/Images/ab190484-225709-ab190484.jpg "Anti-Kappa light chain antibody [RM103] (ab190484)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM103] to Kappa light chain
- Suitable for: WB, ELISA
- Reacts with: Mouse, Recombinant fragment
Overview
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Product name
Anti-Kappa light chain antibody [RM103]
See all Kappa light chain primary antibodies -
Description
Rabbit monoclonal [RM103] to Kappa light chain -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragmentWB Mouse
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Immunogen
Full length protein corresponding to Mouse Kappa light chain. (Mouse IgG).
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Positive control
- Mouse IgG1 kappa.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 48.91% PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab190484 was purified from an animal origin–free and protein-free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM103 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Kappa light chain antibody [RM103] (ab190484)All lanes : Anti-Kappa light chain antibody [RM103] (ab190484) at 0.2 µg/ml
Lane 1 : Non-reduced Mouse IgG1 kappa
Lane 2 : Reduced Mouse IgG1 kappa
Lane 3 : Non-reduced Mouse IgG1 lambda
Lane 4 : Reduced Mouse IgG1 lambda
Lysates/proteins at 0.02 µg per lane.
Predicted band size: 25 kDaab190484 reacted to Non-reduced IgG1 kappa (~150 kDa), and slightly reacted to reduced kappa light chain (~25 kDa).
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![ELISA - Anti-Kappa light chain antibody [RM103] (ab190484)](https://www.abcam.com/ps/products/190/ab190484/Images/ab190484-225835-ab190484E.jpg)
ELISA - Anti-Kappa light chain antibody [RM103] (ab190484)ELISA of mouse immunoglobulins shows ab190484 reacted to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab190484 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.
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![ELISA - Anti-Kappa light chain antibody [RM103] (ab190484)](https://www.abcam.com/ps/products/190/ab190484/Images/ab190484-225836-ab190484E2.JPG)
ELISA - Anti-Kappa light chain antibody [RM103] (ab190484)A titer ELISA of mouse IgG1 kappa. The plate was coated with different amounts of mouse IgG1 kappa. A serial dilution of ab190484 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.
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![Anti-Kappa light chain antibody [RM103] (ab190484)](https://www.abcam.com/ps/products/190/ab190484/Images/ab190484-4-benefits-of-recombinant-antibodies.png)
Anti-Kappa light chain antibody [RM103] (ab190484)
