Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Kappa light chain antibody [EPR5367-8] (ab124727)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5367-8] to Kappa light chain
  • Suitable for: WB, IP, ELISA, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Kappa light chain antibody [EPR5367-8]
    See all Kappa light chain primary antibodies

  • Description

    Rabbit monoclonal [EPR5367-8] to Kappa light chain

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Full length native protein (purified) corresponding to Human Kappa light chain. Purified Human IgA.

  • Positive control

    • IP: Human spleen lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5367-8

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)
    Immunoprecipitation - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)

    Purified ab124727 at 1/50 dilution (2µg) immunoprecipitating Kappa light chain in Human spleen lysate.
    Lane 1 (input): Human spleen lysate 10µg
    Lane 2 (+): ab124727 + Human spleen lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124727 in Human spleen lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 25 kDa

  • Anti-Kappa light chain antibody [EPR5367-8] (ab124727)
    Anti-Kappa light chain antibody [EPR5367-8] (ab124727)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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