Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸ 10 µl 123 964 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Kappa light chain antibody [EPR5367-8] (ab124727)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5367-8] to Kappa light chain
Suitable for: WB, IP, ELISA, IHC-P
Reacts with: Human

Product name

Anti-Kappa light chain antibody [EPR5367-8]
See all Kappa light chain primary antibodies
Description

Rabbit monoclonal [EPR5367-8] to Kappa light chain
Host species

Rabbit

Tested Applications & Species

Application	Species
ELISA	Recombinant fragment
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Full length native protein (purified) corresponding to Human Kappa light chain. Purified Human IgA.
Positive control
- IP: Human spleen lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR5367-8
Isotype

IgG
Research areas

Immunoprecipitation - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)

Purified ab124727 at 1/50 dilution (2µg) immunoprecipitating Kappa light chain in Human spleen lysate.
Lane 1 (input): Human spleen lysate 10µg
Lane 2 (+): ab124727 + Human spleen lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124727 in Human spleen lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 25 kDa
Anti-Kappa light chain antibody [EPR5367-8] (ab124727)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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