Anti-Kappa light chain antibody (ab134083)
Key features and details
- Rabbit polyclonal to Kappa light chain
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Kappa light chain antibody
See all Kappa light chain primary antibodies -
Description
Rabbit polyclonal to Kappa light chain -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
corresponding to Human Kappa light chain.
(Peptide available asab216266) -
Positive control
- Human tonsil and Human plasma lysates, Human tonsil tissue
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General notes
Produced under U.S. Patent No. 5,675,063.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody (ab134083)Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling Kappa light chain with ab134083 at 1/250.
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Western blot - Anti-Kappa light chain antibody (ab134083)All lanes : Anti-Kappa light chain antibody (ab134083) at 1/10000 dilution
Lane 1 : Human tonsil lysate
Lane 2 : Human plasma lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-rabbit at 1/2000 dilution
Predicted band size: 12 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
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Western blot - Anti-Kappa light chain antibody (ab134083)All lanes : Anti-Kappa light chain antibody (ab134083) at 1/10000 dilution
Lane 1 : Kappa light chain purified protein
Lane 2 : Lambda light chain purified protein
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-rabbit at 1/2000 dilution
Predicted band size: 12 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
