Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)
![Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)](https://www.abcam.com/ps/products/232/ab232152/Images/ab232152-4-anti-kappa-light-chain-antibody-epr5367-8-immunoprecipitation-spleen-human.jpg "Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5367-8] to Kappa light chain - BSA and Azide free
- Suitable for: ICC/IF, IP, IHC-P, WB, ELISA
- Reacts with: Human
Overview
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Product name
Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free
See all Kappa light chain primary antibodies -
Description
Rabbit monoclonal [EPR5367-8] to Kappa light chain - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, IHC-P, WB, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human Kappa light chain. Purified Human IgA
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Positive control
- IP: Human spleen lysate.
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General notes
Ab232152 is the carrier-free version of ab124727. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab232152 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5367-8 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)
This data was developed using ab124727, the same antibody clone in a different buffer formulation.
Purified ab124727 at 1/50 dilution (2µg) immunoprecipitating Kappa light chain in Human spleen lysate.
Lane 1 (input): Human spleen lysate 10µg
Lane 2 (+): ab124727 + Human spleen lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124727 in Human spleen lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 25 kDa -
![Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)](https://www.abcam.com/ps/products/232/ab232152/Images/ab232152-2-benefits-of-recombinant-antibodies.png)
Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)
