Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
![Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)](https://www.abcam.com/ps/products/134/ab134929/Images/ab134929-149104-anti-human-kappa-chain-antibody-epr5539-71-8-western-blot.jpg "Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5539-71-8] to Human Kappa Chain
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Human Kappa Chain antibody [EPR5539-71-8]
See all Human Kappa Chain primary antibodies -
Description
Rabbit monoclonal [EPR5539-71-8] to Human Kappa Chain -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Other Immunogen Type corresponding to Human Human Kappa Chain.
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Positive control
- Human tonsil, Human spleen, Human thymus, and Human plasma lysates. B cell lymphoma tissue. Human tonsil tissue. ICC: Raji cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5539-71-8 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)All lanes : Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929) at 1/1000 dilution
Lane 1 : Human tonsil lysate
Lane 2 : Human spleen lysate
Lane 3 : Human thymus lysate
Lane 4 : Human plasma lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 49 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)](https://www.abcam.com/ps/products/134/ab134929/Images/ab134929-149103-anti-human-kappa-chain-antibody-epr5539-71-8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)Immunohistochemical analysis of paraffin-embedded B cell lymphoma tissue labelling IgM with ab134929 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)](https://www.abcam.com/ps/products/134/ab134929/Images/ab134929-6-anti-human-kappa-chain-antibody-epr5539-immunocytochemistry-raji-human.jpg)
Immunocytochemistry - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)Immunocytochemistry analysis of Raji (Human Burkitt's lymphoma B lymphocyte? labeling Human Kappa Chain with purified ab134929 at 1/100 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.32 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)](https://www.abcam.com/ps/products/134/ab134929/Images/ab134929-149102-anti-human-kappa-chain-antibody-epr5539-71-8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling IgM with ab134929 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)](https://www.abcam.com/ps/products/134/ab134929/Images/ab134929-149101-anti-human-kappa-chain-antibody-epr5539-71-8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)Immunohistochemical analysis using ab134929 showing positive staining in Normal colon, lymphocytes tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)](https://www.abcam.com/ps/products/134/ab134929/Images/ab134929-149100-anti-human-kappa-chain-antibody-epr5539-71-8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)Immunohistochemical analysis using ab134929 showing positive staining in Normal stomach, lymphocytes tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)](https://www.abcam.com/ps/products/134/ab134929/Images/ab134929-7-benefits-of-recombinant-antibodies.png)
Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
