Anti-Neuroligin 3 antibody [N110/29] (ab186307)
![Anti-Neuroligin 3 antibody [N110/29] (ab186307)](https://www.abcam.com/ps/products/186/ab186307/Images/ab186307-312295-anti-neuroligin-3-antibody-n110-29-immunofluorescence.jpg "Anti-Neuroligin 3 antibody [N110/29] (ab186307)")
Key features and details
- Mouse monoclonal [N110/29] to Neuroligin 3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-Neuroligin 3 antibody [N110/29]
See all Neuroligin 3 primary antibodies -
Description
Mouse monoclonal [N110/29] to Neuroligin 3 -
Host species
Mouse -
Specificity
ab186307 Does not cross-react with Neuroligin 1, 2, or 4 -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Fusion protein corresponding to Rat Neuroligin 3 aa 700 to the C-terminus (C terminal). Accession no AAA97871
Database link: Q62889 -
Positive control
- WB: Mouse brain membrane lysate. ICC/IF: SK-B-NE cells.
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General notes
The clone number has been updated from S110-29 to N110/29, both clone numbers name the same antibody clone.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N110/29 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Neuroligin 3 antibody [N110/29] (ab186307)
4% formaldehyde-fixed SK-N-BE cells stained for Neuroligin 3 (green) using ab186307 at 1/100 dilution in ICC/IF.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 dilution for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes room temperature, 5 minutes room temperature. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Neuroligin 3 Antibody. (D) Composite.
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![Western blot - Anti-Neuroligin 3 antibody [N110/29] (ab186307)](https://www.abcam.com/ps/products/186/ab186307/Images/ab186307-312296-anti-neuroligin-3-antibody-n110-29-western-blot.jpg)
Western blot - Anti-Neuroligin 3 antibody [N110/29] (ab186307)Anti-Neuroligin 3 antibody [N110/29] (ab186307) at 1/200 dilution + Mouse brain membrane lysate at 15 µg
Secondary
Goat Anti-Mouse IgG: HRP at 1/1000 dilution
Predicted band size: 94 kDa
