Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TPT1L + TCTP antibody [EPR13882(B)] (ab182557)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13882(B)] to TPT1L + TCTP
  • Suitable for: IHC-P, WB, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-TPT1L + TCTP antibody [EPR13882(B)]
    See all TPT1L + TCTP primary antibodies

  • Description

    Rabbit monoclonal [EPR13882(B)] to TPT1L + TCTP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat, 293 and HepG2 cell lysates; Human prostate tissue; Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TPT1L + TCTP antibody [EPR13882(B)] (ab182557)
    Western blot - Anti-TPT1L + TCTP antibody [EPR13882(B)] (ab182557)
    All lanes : Anti-TPT1L + TCTP antibody [EPR13882(B)] (ab182557) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 16, 20 kDa
    Observed band size: 22 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPT1L + TCTP antibody [EPR13882(B)] (ab182557)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPT1L + TCTP antibody [EPR13882(B)] (ab182557)

    Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling FLJ44635 + TCTP with ab182557 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-TPT1L + TCTP antibody [EPR13882(B)] (ab182557)
    Flow Cytometry - Anti-TPT1L + TCTP antibody [EPR13882(B)] (ab182557)

    Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling FLJ44635 + TCTP with ab182557 at 1/240 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Immunoprecipitation - Anti-TPT1L + TCTP antibody [EPR13882(B)] (ab182557)
    Immunoprecipitation - Anti-TPT1L + TCTP antibody [EPR13882(B)] (ab182557)

    Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab182557 at 1/50 dilution (Lane 1). Lane 2: Negative control.

    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Anti-TPT1L + TCTP antibody [EPR13882(B)] (ab182557)
    Anti-TPT1L + TCTP antibody [EPR13882(B)] (ab182557)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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