Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PCGF1 antibody [EPR23757-38] (ab259943)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23757-38] to PCGF1
  • Suitable for: WB, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PCGF1 antibody [EPR23757-38]
    See all PCGF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23757-38] to PCGF1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Human
    IP
    Mouse
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa; MCF7; Jurkat; HepG2; THP-1; NIH/3T3; RAW 264.7 and PC-12 whole cell lysate. Flow Cyt: HeLa; NIH/3T3 cells. IP: NIH/3T3 and HeLa whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23757-38

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PCGF1 antibody [EPR23757-38] (ab259943)
    Western blot - Anti-PCGF1 antibody [EPR23757-38] (ab259943)
    All lanes : Anti-PCGF1 antibody [EPR23757-38] (ab259943) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : Jurkat (human t cell leukemia t lymphocyte) whole cell lysate
    Lane 4 : HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate
    Lane 5 : THP-1 (human monocytic leukemia monocyte) whole cell lysate
    Lane 6 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 7 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 8 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 125  seconds

     

  • Flow Cytometry - Anti-PCGF1 antibody [EPR23757-38] (ab259943)
    Flow Cytometry - Anti-PCGF1 antibody [EPR23757-38] (ab259943)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling PCGF1 with ab259943 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-PCGF1 antibody [EPR23757-38] (ab259943)
    Immunoprecipitation - Anti-PCGF1 antibody [EPR23757-38] (ab259943)

    PCGF1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug with ab259943 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259943 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lane 2: ab259943 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259943 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32 seconds

     

  • Flow Cytometry - Anti-PCGF1 antibody [EPR23757-38] (ab259943)
    Flow Cytometry - Anti-PCGF1 antibody [EPR23757-38] (ab259943)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling PCGF1 with ab259943 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-PCGF1 antibody [EPR23757-38] (ab259943)
    Immunoprecipitation - Anti-PCGF1 antibody [EPR23757-38] (ab259943)

    PCGF1 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug with ab259943 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259943 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Lane 2: ab259943 IP in NIH/3T3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259943 in NIH/3T3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes

     

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