Anti-SMC6 antibody (ab241577)
Key features and details
- Rabbit polyclonal to SMC6
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-SMC6 antibody
See all SMC6 primary antibodies -
Description
Rabbit polyclonal to SMC6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human SMC6 aa 1050-1091. The exact sequence is proprietary.
Database link: Q96SB8 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: SMC6 IP in HeLa whole cell lysate. IHC-P: Human prostate carcinoma tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241577 was affinity purified using an epitope specific to SMC6 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-SMC6 antibody (ab241577)All lanes : Anti-SMC6 antibody (ab241577) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 126 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
-
Immunoprecipitation - Anti-SMC6 antibody (ab241577)SMC6 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241577 at 20 µl per reaction. Western blot was performed from the immunoprecipitate using ab241577 at 1/1000 dilution.
Lane 1: ab241577 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
Lysates prepared using NETN lysis buffer.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC6 antibody (ab241577)Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for SMC6 using ab241577 at 1/40 dilution in immunohistochemical analysis. Detection: DAB staining. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody.
