Human CBS knockout HeLa cell line (ab264950)
Properties
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Number of cells
1 x 106 cells/vial, 1 mL -
Viability
~90% -
Adherent /Suspension
Adherent -
Tissue
Cervix -
Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10 -
Antibiotic resistance
Puromycin 1.00µg/ml -
Mycoplasma free
Yes -
Storage instructions
Shipped on Dry Ice. Store in liquid nitrogen. -
Storage buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether -
Research areas
Images
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Western blot - Human CBS knockout HeLa cell line (ab264950)All lanes : Anti-CBS antibody [EPR8579] (ab140600) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CBS knockout HeLa cell lysate
Lane 3 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab140600 observed at 70 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab140600 Anti-CBS antibody [EPR8579] was shown to specifically react with CBS in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264950 (knockout cell lysate ab257203) was used. Wild-type and CBS knockout samples were subjected to SDS-PAGE. ab140600 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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Western blot - Human CBS knockout HeLa cell line (ab264950)All lanes : Anti-CBS antibody [EPR8578] (ab131155) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CBS knockout HeLa cell lysate
Lane 3 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab131155 observed at 70 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab131155 Anti-CBS antibody [EPR8578] was shown to specifically react with CBS in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264950 (knockout cell lysate ab257203) was used. Wild-type and CBS knockout samples were subjected to SDS-PAGE. ab131155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human CBS knockout HeLa cell line (ab264950)Allele-1: 11 bp deletion in exon 5.
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Sanger Sequencing - Human CBS knockout HeLa cell line (ab264950)Allele-2: 1 bp insertion in exon 5.
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Cell Culture - Human CBS knockout HeLa cell line (ab264950)Representative images of CBS knockout HeLa cells, low and high confluency examples (top left and right respectively) and wild-type HeLa cells, low and high confluency (bottom left and right respectively) showing typical adherent, epithelial-like morphology. Images were captured at 10X magnification using a EVOS XL Core microscope.