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Biotin Anti-6X His tag® antibody [HIS.H8] (ab173828)

Price and availability

291 484 ₸

Availability

Order now and get it on Thursday February 25, 2021

Biotin Anti-6X His tag® antibody [HIS.H8] (ab173828)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Biotin Mouse monoclonal [HIS.H8] to 6X His tag®
  • Suitable for: ELISA, IP, ICC/IF, WB
  • Reacts with: Species independent
  • Conjugation: Biotin
  • Isotype: IgG2b

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Overview

  • Product name

    Biotin Anti-6X His tag® antibody [HIS.H8]
    See all 6X His tag® primary antibodies
  • Description

    Biotin Mouse monoclonal [HIS.H8] to 6X His tag®
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: ELISA, IP, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to 6X His tag® aa 1-6.
    Sequence:

    HHHHHH

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • E. coli lysate containing a multi-epitope tagged protein.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    HIS.H8
  • Isotype

    IgG2b
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • His Tag

Images

  • Western blot - Biotin Anti-6X His tag® antibody [HIS.H8] (ab173828)
    Western blot - Biotin Anti-6X His tag® antibody [HIS.H8] (ab173828)
    All lanes : Biotin Anti-6X His tag® antibody [HIS.H8] (ab173828) at 1/1000 dilution

    Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 0.5 µg
    Lane 2 : E. coli lysate containing a multi-epitope tagged protein at 0.25 µg
    Lane 3 : E. coli lysate containing a multi-epitope tagged protein at 0.125 µg
    Lane 4 : E. coli lysate containing a multi-epitope tagged protein at 0.0599999986588955 µg
    Lane 5 : E. coli lysate containing a multi-epitope tagged protein at 0.0299999993294477 µg
    Lane 6 : E. coli lysate containing a multi-epitope tagged protein at 0.0149999996647239 µg

    Secondary
    All lanes : Streptavidin-HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 0.8 kDa



    Note: Predicted MW is for the His tag alone.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Biotin Anti-6X His tag® antibody [HIS.H8] (ab173828)

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    Anti-6X His tag® antibody [JPAR-2] - BSA and Azide free (ab245203)

    Applications: Flow Cyt, IHC, IP, WB

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    Anti-6X His tag® antibody (ab14923)

    Applications: WB

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    Anti-6X His tag® antibody [4D11] (ab5000)

    Applications: Dot, ELISA, Flow Cyt, ICC/IF, IP, WB

  •  
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    Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204)

    Applications: ChIP, Flow Cyt, ICC/IF, IHC-P, IP, WB

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  • Product image

    Anti-6X His tag® antibody [JPAR-2] (ab245114)

    Applications: Flow Cyt, IHC, IP, WB

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    HRP Anti-6X His tag® antibody [4D11] (ab202004)

    Applications: WB

  •  
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    Anti-6X His tag® antibody [EPR20547] - BSA and Azide free (ab232492)

    Applications: ChIP, Flow Cyt, ICC, IHC-P, IP, WB

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