Anti-Nectin 2 antibody [B-C12] - BSA and Azide free (ab269721)
![Anti-Nectin 2 antibody [B-C12] - BSA and Azide free (ab269721)](https://www.abcam.com/ps/products/269/ab269721/Images/ab269721-2-Nectin2Primaryantibodiesab471711.jpg "Anti-Nectin 2 antibody [B-C12] - BSA and Azide free (ab269721)")
Key features and details
- Mouse monoclonal [B-C12] to Nectin 2 - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
-
Product name
Anti-Nectin 2 antibody [B-C12] - BSA and Azide free
See all Nectin 2 primary antibodies -
Description
Mouse monoclonal [B-C12] to Nectin 2 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF Human
-
Immunogen
Tissue, cells or virus corresponding to Human Nectin 2.
-
Positive control
- KG-1 cell line
-
General notes
ab269721 is a carrier free version of ab47171. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes. This product was changed from ascites to tissue culture supernatant on 4th April 2 19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
B-C12 -
Myeloma
P3x63-Ag8.653 -
Isotype
IgG2b -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Nectin 2 antibody (ab269721)
ICC/IF image of ab47171 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47171, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab47171). -
Flow Cytometry - Anti-Nectin 2 antibody (ab269721)
