Anti-CD32 antibody [AT130-5] - Chimeric (ab281897)
![Anti-CD32 antibody [AT130-5] - Chimeric (ab281897)](https://www.abcam.com/ps/products/281/ab281897/Images/ab281897-459026-AntiCD32-antibody-immunocytochemistry-splenocytesmouse.JPG "Anti-CD32 antibody [AT130-5] - Chimeric (ab281897)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [AT130-5] to CD32 - Chimeric
- Suitable for: ICC
- Reacts with: Mouse
Overview
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Product name
Anti-CD32 antibody [AT130-5] - Chimeric
See all CD32 primary antibodies -
Description
Rabbit monoclonal [AT130-5] to CD32 - Chimeric -
Host species
Rabbit -
Tested applications
Suitable for: ICCmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Fusion protein corresponding to Mouse CD32. CD4 domain 3/4 fustion protein
Database link: P08101 -
Positive control
- ICC: Mouse splenocyte cells
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General notes
ab281897 is the chimeric rabbit version of ab281896.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
AT130-5 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry - Anti-CD32 antibody [AT130-5] (ab281897)
Immunofluorescence analysis of paraformaldehyde-fixed, 0.15% Triton permeabilized mouse splenocytes labeling CD32 with ab281897 at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody at 1 µg/ml. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom ab281897, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor®488 secondary antibody.