Anti-IL-5RA antibody [CAL40] (ab237716)
![Anti-IL-5RA antibody [CAL40] (ab237716)](https://www.abcam.com/ps/products/237/ab237716/Images/ab237716-345788-antiIL5ra-antibody-cal40-immunohistochemistry-paraffin-tonsil-human.jpg "Anti-IL-5RA antibody [CAL40] (ab237716)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [CAL40] to IL-5RA
- Suitable for: ELISA, IHC-P
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-IL-5RA antibody [CAL40]
See all IL-5RA primary antibodies -
Description
Rabbit monoclonal [CAL40] to IL-5RA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragmentIHC-P Human
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Immunogen
Synthetic peptide within Human IL-5RA aa 1-350. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q01344 -
Positive control
- IHC-P: Human tonsil tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purity >99%. -
Clonality
Monoclonal -
Clone number
CAL40 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-5RA antibody [CAL40] (ab237716)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IL-5RA with ab237716 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human tonsil is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
The section was incubated with ab237716 for 15 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-5RA antibody [CAL40] (ab237716)](https://www.abcam.com/ps/products/237/ab237716/Images/ab237716-315263-anti-il-5ra-antibody-cal40-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-5RA antibody [CAL40] (ab237716)Formalin-fixed, paraffin-embedded human tonsil tissue stained for IL5RA using ab237716 at 0.125 μg/ml in immunohistochemical analysis.
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![ELISA - Anti-IL-5RA antibody [CAL40] (ab237716)](https://www.abcam.com/ps/products/237/ab237716/Images/ab237716-3-anti-il-5ra-antibody-cal40-elisa-human-il5ra.jpg)
ELISA - Anti-IL-5RA antibody [CAL40] (ab237716)ELISA analysis of Human IL5RA recombinant protein at 1000 ng/mL with ab237716. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody. -
![Anti-IL-5RA antibody [CAL40] (ab237716)](https://www.abcam.com/ps/products/237/ab237716/Images/ab237716-4-benefits-of-recombinant-antibodies.png)
Anti-IL-5RA antibody [CAL40] (ab237716)
