BCP Albumin Assay Kit (ab272526)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Plasma, Serum, Urine
Overview
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Product name
BCP Albumin Assay Kit
See all Albumin kits -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma -
Assay type
Quantitative -
Range
45 µM - 750 µM -
Product overview
BCP Albumin Assay Kit (ab272526) is a simple, direct and automation-ready procedures for measuring albumin concentration in biological samples. BCP Albumin Assay Kit is designed to measure albumin directly in biological samples without any pretreatment. The improved method utilizes bromcresol purple that forms a colored complex specifically with albumin. The intensity of the color, measured at 610nm, is directly proportional to the albumin concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
Sensitive and accurate. Use as little as 20 μL samples. Detection range 0.3 - 5 g/dL (45 - 750 μM) albumin in 96-well plate assay.
Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay for thousands of samples per day.
Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay.
Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein.
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Tested applications
Suitable for: Functional Studiesmore details -
Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 250 tests Albumin Standard 1 x 2ml Reagent 1 x 50ml -
Research areas
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Function
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Post-translational
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- alb
- ALBU_HUMAN
- Albumin (32 AA)
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Images
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Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Example BSA standard curve.