Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Nectin 2 antibody [EPR6717] (ab135246)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6717] to Nectin 2
  • Suitable for: WB, Flow Cyt, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Nectin 2 antibody [EPR6717]
    See all Nectin 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR6717] to Nectin 2

  • Host species

    Rabbit

  • Specificity

    ab135246 does not recognize Isoform Alpha of human Nectin-2.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Nectin 2 aa 500-600 (intracellular). The exact sequence is proprietary.

  • Positive control

    • Flow cyt: HUVEC cells; ICC: HT-29 cells; WB: HT-29 and SW480 cells, mouse and rat brain lysate; IP: SW480 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunoprecipitation - Anti-Nectin 2 antibody [EPR6717] (ab135246)
    Immunoprecipitation - Anti-Nectin 2 antibody [EPR6717] (ab135246)

    Purified ab135246 at 1/20 dilution (1µg) immunoprecipitating Nectin 2 in SW480 whole cell lysate.
    Lane 1 (input): SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab135246 + SW480 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab135246 in SW480 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 70-85 kDa

  • Western blot - Anti-Nectin 2 antibody [EPR6717] (ab135246)
    Western blot - Anti-Nectin 2 antibody [EPR6717] (ab135246)
    Anti-Nectin 2 antibody [EPR6717] (ab135246) at 1/1000 dilution (Purified) + SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 58 kDa
    Observed band size: 70-85 kDa
    why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in the literature (PMID: 15108322, PMID:12445825 and PMID:12913096).

    Blocking Buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-Nectin 2 antibody [EPR6717] (ab135246)
    Western blot - Anti-Nectin 2 antibody [EPR6717] (ab135246)
    All lanes : Anti-Nectin 2 antibody [EPR6717] (ab135246) at 1/1000 dilution (Purified)

    Lane 1 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 58 kDa
    Observed band size: 70-85 kDa why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in the literature (PMID: 15108322, PMID:12445825 and PMID:12913096).

    Blocking Buffer and concentration: 5% NFDM/TBST

  • Immunocytochemistry - Anti-Nectin 2 antibody [EPR6717] (ab135246)
    Immunocytochemistry - Anti-Nectin 2 antibody [EPR6717] (ab135246)

    Immunocytochemistry analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling Nectin 2 with Purified ab135246 at 1:50 dilution (3.06 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-Nectin 2 antibody [EPR6717] (ab135246)
    Flow Cytometry - Anti-Nectin 2 antibody [EPR6717] (ab135246)

    Flow Cytometry analysis of HUVEC (Human umbilical vein endothelial cell) cells labeling Nectin 2 with Purified ab135246 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Anti-Nectin 2 antibody [EPR6717] (ab135246)
    Anti-Nectin 2 antibody [EPR6717] (ab135246)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Nectin 2 antibody [EPR6717] (ab135246)

© 2021 Astana Biomed Group LLP. All rights reserved.