Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Nectin 2 antibody [EPR6717] (ab135246)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6717] to Nectin 2
Suitable for: WB, Flow Cyt, ICC/IF, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Nectin 2 antibody [EPR6717]
See all Nectin 2 primary antibodies
Description

Rabbit monoclonal [EPR6717] to Nectin 2
Host species

Rabbit
Specificity

ab135246 does not recognize Isoform Alpha of human Nectin-2.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human Nectin 2 aa 500-600 (intracellular). The exact sequence is proprietary.
Positive control
- Flow cyt: HUVEC cells; ICC: HT-29 cells; WB: HT-29 and SW480 cells, mouse and rat brain lysate; IP: SW480 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Dissociation constant (K_D)

K_D = 5.30 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6717
Isotype

IgG
Research areas

Immunoprecipitation - Anti-Nectin 2 antibody [EPR6717] (ab135246)

Purified ab135246 at 1/20 dilution (1µg) immunoprecipitating Nectin 2 in SW480 whole cell lysate.
Lane 1 (input): SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab135246 + SW480 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab135246 in SW480 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 70-85 kDa
Western blot - Anti-Nectin 2 antibody [EPR6717] (ab135246)

Anti-Nectin 2 antibody [EPR6717] (ab135246) at 1/1000 dilution (Purified) + SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 58 kDa
Observed band size: 70-85 kDa
why is the actual band size different from the predicted?

The molecular weight observed is consistent with what has been described in the literature (PMID: 15108322, PMID:12445825 and PMID:12913096).

Blocking Buffer and concentration: 5% NFDM/TBST
Western blot - Anti-Nectin 2 antibody [EPR6717] (ab135246)

All lanes : Anti-Nectin 2 antibody [EPR6717] (ab135246) at 1/1000 dilution (Purified)

Lane 1 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 58 kDa
Observed band size: 70-85 kDa why is the actual band size different from the predicted?

The molecular weight observed is consistent with what has been described in the literature (PMID: 15108322, PMID:12445825 and PMID:12913096).

Blocking Buffer and concentration: 5% NFDM/TBST
Immunocytochemistry - Anti-Nectin 2 antibody [EPR6717] (ab135246)

Immunocytochemistry analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling Nectin 2 with Purified ab135246 at 1:50 dilution (3.06 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry - Anti-Nectin 2 antibody [EPR6717] (ab135246)

Flow Cytometry analysis of HUVEC (Human umbilical vein endothelial cell) cells labeling Nectin 2 with Purified ab135246 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Anti-Nectin 2 antibody [EPR6717] (ab135246)

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