Human ECM1 ELISA Kit (ab246524)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 36 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ECM1 ELISA Kit
See all Extracellular matrix protein 1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 24 2.8% Inter-assay Sample n Mean SD CV% Serum 3 3.2% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
36 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 101 97% - 104% Serum 114 104% - 132% Hep Plasma 105 100% - 109% EDTA Plasma 109 100% - 112% Cit plasma 109 104% - 113% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human ECM1 ELISA Kit (ab246524) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ECM1 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ECM1 with 36 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human ECM1 Capture Antibody 1 x 600µl 10X Human ECM1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human ECM1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. -
Tissue specificity
Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin; wide expression is observed throughout the dermis with minimal expression in the epidermis. -
Involvement in disease
Defects in ECM1 are the cause of lipoid proteinosis (LiP) [MIM:247100]; also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- ECM 1
- Ecm1
- ECM1_HUMAN
see all -
Database links
- Entrez Gene: 1893 Human
- Omim: 602201 Human
- SwissProt: Q16610 Human
- Unigene: 81071 Human
Images
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Other - Human ECM1 ELISA Kit (ab246524)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human ECM1 standard curve in Sample Diluent NS.The ECM1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native ECM1 in human serum, plasma.The concentrations of ECM1 were measured in duplicates, interpolated from the ECM1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:1000, plasma (citrate) 1:2000, plasma (EDTA) 1:2000, and plasma (heparin) 1:1000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ECM1 concentration was determined to be 4064 ng/mL in serum, 9512 ng/mL in plasma (citrate), 10307 ng/mL in plasma (EDTA), and 2989 ng/ml in plasma heparin.
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Interpolated concentrations of native ECM1 in cell culture supernatant.The concentrations of ECM1 were measured in duplicate, interpolated from the ECM1 standard curves and corrected for sample dilution. Undiluted samples are as follows: MDA-MB-435S supernatant 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ECM1 concentration was determined to be 141 ng/mL.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ECM1 concentration was determined to be 2862 ng/mL with a range of 1028 – 4859 ng/mL.
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Sandwich ELISA - Human ECM1 ELISA Kit (ab246524)To learn more about the advantages of recombinant antibodies see here.
