Anti-Nectin 2 antibody [B-C12] (ab47171)
![Anti-Nectin 2 antibody [B-C12] (ab47171)](https://www.abcam.com/ps/products/47/ab47171/Images/ab47171_FCS.jpg "Anti-Nectin 2 antibody [B-C12] (ab47171)")
Key features and details
- Mouse monoclonal [B-C12] to Nectin 2
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Nectin 2 antibody [B-C12]
See all Nectin 2 primary antibodies -
Description
Mouse monoclonal [B-C12] to Nectin 2 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
KG-1 cell line
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Positive control
- KG-1 cell line
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General notes
This product was changed from ascites to tissue culture supernatant on 4th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Constituent: PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
B-C12 -
Myeloma
x63-Ag8.653 -
Isotype
IgG2b -
Research areas
Images
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Flow Cytometry - Anti-Nectin 2 antibody [B-C12] (ab47171)
A typical staining pattern with ab47171 monoclonal antibody of KG-1 cell line.
This image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-Nectin 2 antibody [B-C12] (ab47171)](https://www.abcam.com/ps/products/47/ab47171/Images/Nectin-2-Primary-antibodies-ab47171-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nectin 2 antibody [B-C12] (ab47171)ICC/IF image of ab47171 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47171, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
