Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LILRB1 antibody [EPR22861-6] - BSA and Azide free (ab255986)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22861-6] to LILRB1 - BSA and Azide free
  • Suitable for: IP, WB, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-LILRB1 antibody [EPR22861-6] - BSA and Azide free
    See all LILRB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22861-6] to LILRB1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Human PBMC cells. Flow Cyt: Human PBMC and HEK-293T cells. IP: IM-9 cell lysate.

  • General notes

    Ab255986 is the carrier-free version of ab238145. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255986 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-LILRB1 antibody [EPR22861-6] - BSA and Azide free (ab255986)
    Immunoprecipitation - Anti-LILRB1 antibody [EPR22861-6] - BSA and Azide free (ab255986)

    LILRB1 was immunoprecipitated from 0.35 mg IM-9 (Human multiple myeloma B Lymphoblast) whole cell lysate with ab238145 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238145 1/1000 dilution (0.48 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

    Lane 1: IM-9 (Human multiple myeloma B Lymphoblast) whole cell lysate 10μg
    Lane 2: ab238145 IP in IM-9 whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238145 in IM-9 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238145).

  • Immunocytochemistry/ Immunofluorescence - Anti-LILRB1 antibody [EPR22861-6] - BSA and Azide free (ab255986)
    Immunocytochemistry/ Immunofluorescence - Anti-LILRB1 antibody [EPR22861-6] - BSA and Azide free (ab255986)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized human PBMC (human primary peripheral blood mononuclear cell) cells labeling LILRB1 with ab238145 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in subsets of human PBMC is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)  was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238145).

  • Flow Cytometry - Anti-LILRB1 antibody [EPR22861-6] - BSA and Azide free (ab255986)
    Flow Cytometry - Anti-LILRB1 antibody [EPR22861-6] - BSA and Azide free (ab255986)

    Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with myc-tagged LILRB2 expression vector (Left) / HEK-293T transfected with myc-tagged LILRB1 expression vector (Right) cells labeling LILRB1 with ab238145 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with rabbit IgG (black) or ab238145 (red). Then fixed with 2% PFA followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647. Gated on myc+ population.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238145).

  • Flow Cytometry - Anti-LILRB1 antibody [EPR22861-6] - BSA and Azide free (ab255986)
    Flow Cytometry - Anti-LILRB1 antibody [EPR22861-6] - BSA and Azide free (ab255986)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling LILRB1 with ab238145 at 1/500 dilution (0.1μg)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody. Cells were stained with anti-CD11b conjugated to BV421. Then stained with rabbit IgG (Left) or ab238145 (Right). Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238145).

  • Anti-LILRB1 antibody [EPR22861-6] - BSA and Azide free (ab255986)
    Anti-LILRB1 antibody [EPR22861-6] - BSA and Azide free (ab255986)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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