Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Thursday March 04, 2021

Anti-LILRB1 antibody [EPR21007] (ab229186)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21007] to LILRB1
Suitable for: IHC-P
Reacts with: Human

Product name

Anti-LILRB1 antibody [EPR21007]
See all LILRB1 primary antibodies
Description

Rabbit monoclonal [EPR21007] to LILRB1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: lung cancer and liver tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21007
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB1 antibody [EPR21007] (ab229186)

Immunohistochemical analysis of paraffin-embedded human NK/T-cell lymphoma tissue labeling LILRB1 with ab229186 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic and membranous staining in human NK/T-cell lymphoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-LILRB1 antibody [EPR21007] (ab229186)

All lanes : Anti-LILRB1 antibody [EPR21007] (ab229186) at 1/1000 dilution

Lane 1 : Human fetal spleen lysate
Lane 2 : Human tonsil lysate
Lane 3 : IM-9 (human multiple myeloma B Lymphoblast cell line) whole cell lysate
Lane 4 : NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 71 kDa
Observed band size: 90-110 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

High LILRB1 expression not only in lymphoid tissues but also in NK (NK-92) and lymphoblastoid (IM-9) cell lines (PMID: 22844324).

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB1 antibody [EPR21007] (ab229186)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling LILRB1 with ab229186 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic and membranous staining in tumor-infiltrating immunocytes is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB1 antibody [EPR21007] (ab229186)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling LILRB1 with ab229186 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic and membranous staining in Kupffer cells of human liver is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-LILRB1 antibody [EPR21007] (ab229186)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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