Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (ab224197)
![Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (ab224197)](https://www.abcam.com/ps/products/224/ab224197/Images/ab224197-319044-anti-ifn-gamma-receptor-beta-af-1-antibody-epr22171-flow-cytometry.jpg "Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (ab224197)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22171] to IFN gamma Receptor beta/AF-1
- Suitable for: ELISA, ICC/IF, Flow Cyt
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171]
See all IFN gamma Receptor beta/AF-1 primary antibodies -
Description
Rabbit monoclonal [EPR22171] to IFN gamma Receptor beta/AF-1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragmentFlow Cyt HumanICC/IF Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HEK-293T cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct. Flow Cyt: HEK-293T cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22171 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (ab224197)
Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct cell line labeling IFN gamma Receptor beta/AF-1 with ab224197 at 1/400 dilution.
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Cells were surface stained with isotype control Rabbit monoclonal IgG (ab172730) (Left panel) or IFN gamma Receptor beta/AF-1 (Right panel), then co-stained with Alexa Fluor® 647 conjugated-Myc-tag antibody.
Gated on viable cells.
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![Immunocytochemistry/ Immunofluorescence - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (ab224197)](https://www.abcam.com/ps/products/224/ab224197/Images/ab224197-319043-anti-ifn-gamma-receptor-beta-af-1-antibody-epr22171-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (ab224197)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct labeling IFN gamma Receptor beta/AF-1 with ab224197 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing positive staining in HEK-293T cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct. The nuclear counterstain is DAPI (blue).
Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red) and Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) (white).
The negative control is the secondary antibody only. -
![ELISA - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (ab224197)](https://www.abcam.com/ps/products/224/ab224197/Images/ab224197-3-anti-ifn-gamma-receptor-betaaf-1-antibody-epr22171-elisa-human-ifngr2.jpg)
ELISA - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (ab224197)ELISA analysis of Human IFNGR2 recombinant protein at 1000 ng/mL with ab224197. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody. -
![Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (ab224197)](https://www.abcam.com/ps/products/224/ab224197/Images/ab224197-4-benefits-of-recombinant-antibodies.png)
Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (ab224197)
