Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (ab240559)
![Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (ab240559)](https://www.abcam.com/ps/products/240/ab240559/Images/ab240559-320594-anti-ifn-gamma-receptor-beta-af-1-antibody-epr22171-flow-cytometry.jpg "Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (ab240559)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22171] to IFN gamma Receptor beta/AF-1 - BSA and Azide free
- Suitable for: ELISA, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free
See all IFN gamma Receptor beta/AF-1 primary antibodies -
Description
Rabbit monoclonal [EPR22171] to IFN gamma Receptor beta/AF-1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HEK-293T cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct.
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General notes
Ab240559 is the carrier-free version of ab224197. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240559 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22171 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (ab240559)
Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct cell line labeling IFN gamma Receptor beta/AF-1 with ab224197 at 1/400 dilution.
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Cells were surface stained with isotype control Rabbit monoclonal IgG (ab172730) (Left panel) or IFN gamma receptor beta/AF-1 (Right panel), then co-stained with Alexa Fluor® 647 conjugated-Myc-tag antibody.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224197).
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![Immunocytochemistry/ Immunofluorescence - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (ab240559)](https://www.abcam.com/ps/products/240/ab240559/Images/ab240559-319065-anti-ifn-gamma-receptor-beta-af-1-antibody-epr22171-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (ab240559)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct labeling IFN gamma Receptor beta/AF-1 with ab224197 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing positive staining in HEK-293T cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct. The nuclear counterstain is DAPI (blue).
Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red) and Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) (white).
The negative control is the secondary antibody only.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab224197).
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![ELISA - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (ab240559)](https://www.abcam.com/ps/products/240/ab240559/Images/ab240559-3-anti-ifn-gamma-receptor-betaaf-1-antibody-epr22171-elisa-human-ifngr2.jpg)
ELISA - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (ab240559)This data was developed using ab224197, the same antibody clone in a different buffer formulation.
ELISA analysis of Human IFNGR2 recombinant protein at 1000 ng/mL with ab224197. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody. -
![Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (ab240559)](https://www.abcam.com/ps/products/240/ab240559/Images/ab240559-4-benefits-of-recombinant-antibodies.png)
Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (ab240559)
