Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (ab240559)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22171] to IFN gamma Receptor beta/AF-1 - BSA and Azide free
  • Suitable for: ELISA, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free
    See all IFN gamma Receptor beta/AF-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22171] to IFN gamma Receptor beta/AF-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HEK-293T cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct.

  • General notes

    Ab240559 is the carrier-free version of ab224197. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240559 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (ab240559)
    Flow Cytometry - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (ab240559)

    Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct cell line labeling IFN gamma Receptor beta/AF-1 with ab224197 at 1/400 dilution.

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    Cells were surface stained with isotype control Rabbit monoclonal IgG (ab172730) (Left panel) or IFN gamma receptor beta/AF-1 (Right panel), then co-stained with Alexa Fluor® 647 conjugated-Myc-tag antibody.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224197).

  • Immunocytochemistry/ Immunofluorescence - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (ab240559)
    Immunocytochemistry/ Immunofluorescence - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (ab240559)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct labeling IFN gamma Receptor beta/AF-1 with ab224197 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing positive staining in HEK-293T cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct. The nuclear counterstain is DAPI (blue).
    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red) and Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) (white).
    The negative control is the secondary antibody only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab224197).

  • ELISA - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (ab240559)
    ELISA - Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (ab240559)

    This data was developed using ab224197, the same antibody clone in a different buffer formulation.

    ELISA analysis of Human IFNGR2 recombinant protein at 1000 ng/mL with ab224197. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
  • Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (ab240559)
    Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (ab240559)

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