Anti-IFN gamma Receptor beta/AF-1 antibody (ab77246)
Key features and details
- Rabbit polyclonal to IFN gamma Receptor beta/AF-1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-IFN gamma Receptor beta/AF-1 antibody
See all IFN gamma Receptor beta/AF-1 primary antibodies -
Description
Rabbit polyclonal to IFN gamma Receptor beta/AF-1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human IFN gamma Receptor beta/AF-1 aa 250 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab90070) -
Positive control
- This antibody gave a positive signal in the following lysates: Tissues: Human spinal cord; Mouse liver; Human skeletal muscle; Mouse kidney. Whole cell: Hela nuclear; F9; A431
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General notes
Previously labelled as IFN gamma Receptor beta.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseHumanAll applications CowPigBaboonDeerApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 38 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 38 kDa).Target
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Function
Part of the receptor for interferon gamma. Required for signal transduction. This accessory factor is an integral part of the IFN-gamma signal transduction pathway and is likely to interact with GAF, JAK1, and/or JAK2. -
Involvement in disease
Defects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. -
Sequence similarities
Belongs to the type II cytokine receptor family.
Contains 2 fibronectin type-III domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3460 Human
- Entrez Gene: 15980 Mouse
- Omim: 147569 Human
- SwissProt: P38484 Human
- Unigene: 634632 Human
- Unigene: 249364 Mouse
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Alternative names
- AF 1 antibody
- AF-1 antibody
- AF1 antibody
see all
Images
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Western blot - Anti-IFN gamma Receptor beta/AF-1 antibody (ab77246)All lanes : Anti-IFN gamma Receptor beta/AF-1 antibody (ab77246) at 1 µg/ml
Lane 1 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 4 : F9 (Mouse embryonic carcinoma cell line)
Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 6 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 7 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 16 kDa, 48 kDa (possible cleavage fragment), 77 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Interferon-gamma receptor beta chain has a predicted molecular weight of 38 kDa, however it is extensively glycosylated and has been shown to run in the 49 kDa region (SwissProt data). The bands present at 48 kDa are representative of the protein minus its 27 amino acid signal peptide. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Datasheets and documents
References (6)
ab77246 has been referenced in 6 publications.
- Chen J et al. Long non-coding RNA-HOTAIR promotes the progression of sepsis by acting as a sponge of miR-211 to induce IL-6R expression. Exp Ther Med 18:3959-3967 (2019). PubMed: 31656541
- Bullock BL et al. Tumor-intrinsic response to IFN? shapes the tumor microenvironment and anti-PD-1 response in NSCLC. Life Sci Alliance 2:N/A (2019). PubMed: 31133614
- Zhang S et al. Swiprosin-1 deficiency impairs macrophage immune response of septic mice. JCI Insight 3:N/A (2018). PubMed: 29415882
- Ma X et al. Alendronate promotes osteoblast differentiation and bone formation in ovariectomy-induced osteoporosis through interferon-ß/signal transducer and activator of transcription 1 pathway. Exp Ther Med 15:182-190 (2018). PubMed: 29375681
- Bi D et al. Effects of leukotriene B4 on interleukin-32, interferon-? and chemokines in rats with rheumatoid arthritis. Exp Ther Med 14:2925-2930 (2017). PubMed: 28912850
- Ionescu VA et al. Cultured adult porcine astrocytes and microglia express functional interferon-? receptors and exhibit toxicity towards SH-SY5Y cells. Brain Res Bull : (2010). ICC/IF ; Pig . PubMed: 21185917
Images
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Western blot - Anti-IFN gamma Receptor beta/AF-1 antibody (ab77246)All lanes : Anti-IFN gamma Receptor beta/AF-1 antibody (ab77246) at 1 µg/ml
Lane 1 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 4 : F9 (Mouse embryonic carcinoma cell line)
Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 6 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 7 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 16 kDa, 48 kDa (possible cleavage fragment), 77 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Interferon-gamma receptor beta chain has a predicted molecular weight of 38 kDa, however it is extensively glycosylated and has been shown to run in the 49 kDa region (SwissProt data). The bands present at 48 kDa are representative of the protein minus its 27 amino acid signal peptide. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
