Recombinant human EPO-R protein (Fc Chimera) (ab84001)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human EPO-R protein (Fc Chimera)
See all EPO-R proteins and peptides -
Biological activity
The ED50 of ab84001 is typically 0.15-2.5 ng/ml as measured by its ability to neutralize EPO-mediated proliferation of TF-1 cells. -
Purity
> 95 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
Theoretical sequence: APPPNLPDPKFESKAALLAARGPEELLCFTERLEDLVCF WEEAASAGV GPGNYSFSYQLEDEPWKLCRLHQAPTARGAVRFWCSLPT ADTSSFVPL ELRVTAASGAPRYHRVIHINEVVLLDAPVGLVARLADES GHVVLRWLP PPETPMTSHIRYEVDVSAGNGAGSVQRVEILEGRTECVL SNLRGRTRY TFAVRARMAEPSFGGFWSAWSEPVSLLTPSDLDPRIPKV DKKVEPKSC DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEV TCVVVDVSH EDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLT VLHQDWLNG KEYKCRVSNKALPAPIEKTISKAKGQPREPQVYTLPPSR DELTKNQVS LTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGS FFLYSKLTV DKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK -
Amino acids
25 to 250 -
Additional sequence information
Encodes the signal peptide & extracellular domain of human Erythropoietin receptor (EPO R, aa 1-250) fused to the Fc region IgG1 (aa 93-330). Chimeric protein was expressed in modified 293 cells.
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Preparation and Storage
- EPO R
- EPO Receptor
- EPO-R
Images
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SDS-PAGE - Recombinant human EPO-R protein (Fc Chimera) (ab84001)Lane 1 – MW markers; Lane 2 – ab84001 ; Lane 3 – ab84001 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab84001 treated with a glycosidase cocktail to remove potential N- and O linked glycans. 10 μg of protein was loaded per lane. Gel was stained with Deep Purple™.
Appearance of additional band at lower MW after treatment with PNGase F indicates the presence of N-linked glycans. A possible subsequent drop in MW after treatment with a glycosidase cocktail indicates O-linked glycans may be present. Additional high MW bands in lane 4 are glycosidase enzymes.
