Human FCGRT (FCRN) knockout HEK293T cell pellet (ab279051)
Overview
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Product name
Human FCGRT (FCRN) knockout HEK293T cell pellet
See all FCGRT/FCRN kits -
Product overview
Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.
Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation. -
Parental Cell Line
HEK293T -
Organism
Human -
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 3. -
Passage number
Knockout validation
Sanger Sequencing, Western Blot (WB)Notes
Pellet size: 5 million cells/vial.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Tested applications
Suitable for: WBmore detailsProperties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human FCGRT knockout HEK293T cell pellet 1 vial Human wild-type HEK293T cell pellet 1 vial -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 11, 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 15, 20 TH01: 7, 9.3 TPOX: 11, 12 CSF1PO: 12
Target
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Function
Binds to the Fc region of monomeric immunoglobulins gamma. Mediates the uptake of IgG from milk. Possible role in transfer of immunoglobulin G from mother to fetus. -
Sequence similarities
Belongs to the immunoglobulin superfamily. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- Alpha chain
- Fc fragment of IgG, receptor transporter, alpha
- FCGRN_HUMAN
see all
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human FCGRT knockout HEK293T cell pellet 1 vial Human wild-type HEK293T cell pellet 1 vial -
Research areas
-
Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 11, 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 15, 20 TH01: 7, 9.3 TPOX: 11, 12 CSF1PO: 12
Images
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Western blot - Human FCGRT (FCRN) knockout HEK293T cell pellet (ab279051)
Lane 1: Wild-type A549 cell lysate (20 µg)
Lane 2: FCGRT knockout A549 cell lysate (20 µg)
Lane 3: HEK293 cell lysate (20 µg)
Lane 4: Lncap cell lysate (20 µg)
Lanes 1-4: Merged signal (red and green). Green - ab228975 observed at 40 kDa. Red - loading control ab7291 observed at 50 kDa.
ab228975 Anti-FCGRT/FCRN antibody [EPR22627-25] was shown to specifically react with FCGRT/FCRN in wild-type A549 cells. Loss of signal was observed when knockout cell line ab265075 (knockout cell lysate ab257435) was used. Wild-type and FCGRT/FCRN knockout samples were subjected to SDS-PAGE. ab228975 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human FCGRT (FCRN) knockout HEK293T cell pellet (ab279051)Homozygous: 14 bp deletion in exon 3
