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Human FCGRT (FCRN) knockout HEK293T cell pellet (ab279051)

Overview

  • Product name

    Human FCGRT (FCRN) knockout HEK293T cell pellet
    See all FCGRT/FCRN kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HEK293T

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 3.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    Binds to the Fc region of monomeric immunoglobulins gamma. Mediates the uptake of IgG from milk. Possible role in transfer of immunoglobulin G from mother to fetus.

  • Sequence similarities

    Belongs to the immunoglobulin superfamily.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Alternative names

    • Alpha chain
    • Fc fragment of IgG, receptor transporter, alpha
    • FCGRN_HUMAN
    • FCGRT
    • FcRn
    • FcRn alpha chain
    • FCRN, alpha chain
    • IgG Fc fragment receptor transporter alpha chain
    • IgG Gc receptor
    • IgG receptor FcRn large subunit p51
    • IgG receptor FcRn large subunit p51 precursor
    • Immunoglobulin receptor, intestinal, heavy chain
    • Neonatal Fc receptor
    see all

Properties

Images

  • Western blot - Human FCGRT (FCRN) knockout HEK293T cell pellet (ab279051)
    Western blot - Human FCGRT (FCRN) knockout HEK293T cell pellet (ab279051)

    Lane 1: Wild-type A549 cell lysate (20 µg)

    Lane 2: FCGRT knockout A549 cell lysate (20 µg)

    Lane 3: HEK293 cell lysate (20 µg)

    Lane 4: Lncap cell lysate (20 µg)

    Lanes 1-4: Merged signal (red and green). Green - ab228975 observed at 40 kDa. Red - loading control ab7291 observed at 50 kDa.

    ab228975 Anti-FCGRT/FCRN antibody [EPR22627-25] was shown to specifically react with FCGRT/FCRN in wild-type A549 cells. Loss of signal was observed when knockout cell line ab265075 (knockout cell lysate ab257435) was used. Wild-type and FCGRT/FCRN knockout samples were subjected to SDS-PAGE. ab228975 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human FCGRT (FCRN) knockout HEK293T cell pellet (ab279051)
    Sanger Sequencing - Human FCGRT (FCRN) knockout HEK293T cell pellet (ab279051)
    Homozygous: 14 bp deletion in exon 3

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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