Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LILRB1 antibody [EPR11256] (ab170909)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11256] to LILRB1
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-LILRB1 antibody [EPR11256]
    See all LILRB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11256] to LILRB1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human LILRB1 aa 600 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8NHL6

  • Positive control

    • WB: THP-1, A431 and Raji cell lysates. IHC-P: human tonsil tissue. ICC/IF: Jurkat cells. Flow Cyt: THP-1 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11256

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB1 antibody [EPR11256] (ab170909)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB1 antibody [EPR11256] (ab170909)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling LILRB1 with purified ab170909 at 1/2000 dilution (0.73 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-LILRB1 antibody [EPR11256] (ab170909)
    Western blot - Anti-LILRB1 antibody [EPR11256] (ab170909)
    All lanes : Anti-LILRB1 antibody [EPR11256] (ab170909) at 1/10000 dilution (Purified)

    Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
    Lane 2 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 71 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-LILRB1 antibody [EPR11256] (ab170909)
    Immunocytochemistry/ Immunofluorescence - Anti-LILRB1 antibody [EPR11256] (ab170909)

    Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling LILRB1 with purified ab170909 at 1/250 dilution (5.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-LILRB1 antibody [EPR11256] (ab170909)
    Flow Cytometry - Anti-LILRB1 antibody [EPR11256] (ab170909)

    Flow Cytometry analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling LILRB1 with purified ab170909 at 1/150 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-LILRB1 antibody [EPR11256] (ab170909)
    Western blot - Anti-LILRB1 antibody [EPR11256] (ab170909)
    All lanes : Anti-LILRB1 antibody [EPR11256] (ab170909) at 1/1000 dilution ((unpurified))

    Lane 1 : A431 cell lysate
    Lane 2 : THP-1 cell lysate
    Lane 3 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 71 kDa

  • Anti-LILRB1 antibody [EPR11256] (ab170909)
    Anti-LILRB1 antibody [EPR11256] (ab170909)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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