Human ROBO4 Antibody Pair is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human ROBO4 in sandwich ELISAs and many other pair-based applications.

The pair can be used in variety of assays and platforms including but not limited to:

     - Sandwich ELISA
     - FRET/TR-FRET/HTR
     - Meso Scale Discovery^® (MSD^®)
     - Luminex^® and bead-based assays
     - AlphaLISA^®/AlphaScreen^®
     - DELFIA^® immunoassays
     - Simoa^® and Single Molecule Counting (SMC™) immunoassays
     - Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

     - Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
     - Concentration of ~1 mg/ml as measured by the protein A280 method.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

 Antibody properties:

 Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

 Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

 Concentration: ~1 mg/ml

 Storage buffer: 100% PBS

 Form: Liquid

 Isotype: IgG

 Recombinant monoclonal antibodies offer several advantages including:

     - High batch-to-batch consistency and reproducibility
     - Improved sensitivity and specificity
     - Long-term security of supply
     - Animal-free production

For more information see here.

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
Luminex is a trademark of Luminex Corporation, registered in the US and other countries.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

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• Extracellular Matrix

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• Neurology process
• Growth and Development
• Axonal Guidance Proteins

• Neuroscience
• Neurology process
• Neurogenesis

• Cancer
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• Angiogenesis
• Other