Anti-Histone H3 (acetyl K115) antibody (ab240888)
Key features and details
- Rabbit polyclonal to Histone H3 (acetyl K115)
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (acetyl K115) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (acetyl K115) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanWB Human
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Immunogen
Synthetic peptide within Human Histone H3 (acetyl K115). The exact sequence is proprietary.
Database link: P68431 -
Positive control
- WB: HeLa, A549, HEK-293 and K562, treated with 30 mM sodium butyrate for 4 hours, whole cell lysates. ICC: HeLa cells (treated with 30 mM sodium butyrate for 4 hours).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H3 (acetyl K115) antibody (ab240888)All lanes : Anti-Histone H3 (acetyl K115) antibody (ab240888) at 1/100 dilution
Lane 1 : Hela (human epithelial cell line from cervix adenocarcinoma) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr
Lane 5 : Hela whole cell lysate, untreated (-)
Lane 6 : HEK-293 whole cell lysate, untreated (-)
Lane 7 : A549 whole cell lysate, untreated (-)
Lane 8 : K562 whole cell lysate, untreated (-)
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 15 kDa
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Immunocytochemistry - Anti-Histone H3 (acetyl K115) antibody (ab240888)HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) stained for Histone H3 (acetyl K115) using ab240888 at 1/4 dilution in ICC.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight.
The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
