Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD226 antibody [BLR063G] - BSA free (ab264415)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR063G] to CD226 - BSA free
  • Suitable for: IP, WB, IHC-P, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-CD226 antibody [BLR063G] - BSA free
    See all CD226 primary antibodies

  • Description

    Rabbit monoclonal [BLR063G] to CD226 - BSA free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human CD226 aa 19-229 (extracellular). The exact sequence is proprietary. NP_006557.2.
    Database link: Q15762

  • Positive control

    • IHC-P: Human lung carcinoma tissue. ICC: Jurkat and HH cells. WB: SUP-M2, HH and Jurkat whole cell lysates. IP: HH cell lysate.

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc

     

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [BLR063G] - BSA free (ab264415)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 antibody [BLR063G] - BSA free (ab264415)

    Formalin-fixed, paraffin embedded human lung carcinoma tissue stained for CD226 using ab264415 at 1/200 dilution in immunohistochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

  • Immunocytochemistry - Anti-CD226 antibody [BLR063G] - BSA free (ab264415)
    Immunocytochemistry - Anti-CD226 antibody [BLR063G] - BSA free (ab264415)

    Formalin-fixed, paraffin-embedded Jurkat (human T cell leukemia cell line from peripheral blood) cells stained for CDD226 using ab264415 at 1/200 dilution in imunocytochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

  • Western blot - Anti-CD226 antibody [BLR063G] - BSA free (ab264415)
    Western blot - Anti-CD226 antibody [BLR063G] - BSA free (ab264415)
    All lanes : Anti-CD226 antibody [BLR063G] - BSA free (ab264415) at 1/1000 dilution

    Lane 1 : RT4 (human urinary bladder cancer cell line) whole cell lysate
    Lane 2 : SUP-M2 whole cell lysate
    Lane 3 : U-251 MG (human brain glioma cell line) whole cell lysate
    Lane 4 : HH whole cell lysate
    Lane 5 : MJ whole cell lysate
    Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 7 : HDLM-2 whole cell lysate
    Lane 8 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 39 kDa


    Exposure time: 3 seconds


    Lysates prepared using NETN lysis buffer.

  • Immunoprecipitation - Anti-CD226 antibody [BLR063G] - BSA free (ab264415)
    Immunoprecipitation - Anti-CD226 antibody [BLR063G] - BSA free (ab264415)

    CD226 was immunoprecipitated from HH whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264415 at 6 µl per reaction. Western blot was performed on the immunoprecipitates using ab264415 at 1/1000 dilution.

    Lane 1: ab264415 IP in HH cell lysate.

    Lane 2: Control IgG IP in HH cell lysate.

    Detection: Chemiluminescence with an exposure time of 3 seconds.

    Lysates prepared using NETN lysis buffer.

  • Immunocytochemistry - Anti-CD226 antibody [BLR063G] - BSA free (ab264415)
    Immunocytochemistry - Anti-CD226 antibody [BLR063G] - BSA free (ab264415)

    Formalin-fixed, paraffin-embedded HH cells stained for CDD226 using ab264415 at 1/200 dilution in imunocytochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

  • Anti-CD226 antibody [BLR063G] - BSA free (ab264415)
    Anti-CD226 antibody [BLR063G] - BSA free (ab264415)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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