Anti-CD226 antibody [EPR21245] (ab245186)
![Anti-CD226 antibody [EPR21245] (ab245186)](https://www.abcam.com/ps/products/245/ab245186/Images/ab245186-336991-anti-cd226-antibody-epr21245-flow-cytometry.jpg "Anti-CD226 antibody [EPR21245] (ab245186)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21245] to CD226
- Suitable for: Flow Cyt
- Reacts with: Mouse
Overview
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Product name
Anti-CD226 antibody [EPR21245]
See all CD226 primary antibodies -
Description
Rabbit monoclonal [EPR21245] to CD226 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
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Immunogen
Recombinant fragment. This information is considered to be commercially sensitive.
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Positive control
- Flow Cyt: C57BL/6 mouse splenocytes
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General notes
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR21245 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-CD226 antibody [EPR21245] (ab245186)
C57BL/6 mouse splenocytes stained with ab245186 (right) or chimeric Rabbit IgG (monoclonal) (left). C57BL/6 Mouse splenocytes were incubated for 30min on ice in 1x PBS containing 10ug/ml anti CD16/32 antibody and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab245186) or chimeric Rabbit IgG Isotype (1x106 in 100ul at 1ug/ml) for 30 min on ice.
The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150081) was used at 1/2000 dilution for 30 min on ice.The cells were simultaneously stained with CD3 Alexa® 700 conjugated antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
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![Anti-CD226 antibody [EPR21245] (ab245186)](https://www.abcam.com/ps/products/245/ab245186/Images/ab245186-2-benefits-of-recombinant-antibodies.png)
Anti-CD226 antibody [EPR21245] (ab245186)
