Recombinant human BDNF protein (Animal Free) (ab222178)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, HPLC
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C long term. Avoid freeze / thaw cycle.
Constituent: 0.1% Trifluoroacetic acid
Lyophilized from a sterile 0.2 micron filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionCentrifuge vial before opening. Suspend the product by gently pipetting sterile water down the sides of the vial to a final concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Upon reconstitution, a small amount of visible precipitate can be expected. A 10% overfill has been added to the total material vialed to compensate for this loss. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaw.
Images
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SDS-PAGE - Recombinant human BDNF protein (Animal Free) (ab222178)
1 µg ab222178 analyzed on a 4-20% Tris-Glycine gel, stained with Coomassie Blue.
Lane 1: Non-reducing conditions.
Lane 2: Reducing conditions.
ab222178 is a non-covalently linked homodimer with a predicted molecular weight of 27.3 kDa. Each monomer has a predicted molecuar weight of 13.6 kDa.
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Functional Studies - Recombinant human BDNF protein (Animal Free) (ab222178)ab222178 induced proliferation of C6 (rat glial tumor cell line) cells.
