Rat beta NGF ELISA Kit (ab193736)
Key features and details
- Sensitivity: 15 pg/ml
- Range: 15 pg/ml - 15000 pg/ml
- Sample type: Cell Lysate, Tissue Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat beta NGF ELISA Kit
See all Beta nerve growth factor kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell Lysate, Tissue Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
15 pg/ml -
Range
15 pg/ml - 15000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell Lysate = 98.06 69% - 108% Tissue Lysate = 99.34 70% - 121% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Abcam’s beta NGF Rat ELISA Kit (ab193736) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat beta NGF in cell lysate and tissue lysate.
This assay employs an antibody specific for rat beta NGF coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures beta NGF present in the samples. The wells are washed and biotinylated anti-rat beta NGF antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of beta NGF bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 2X Cell Lysis Buffer 1 x 5ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml 5X Sample Diluent Buffer 1 x 10ml Biotinylated rat beta-NGF detection antibody (lyophilized) 2 vials Pre-coated rat beta-NGF Microplate (12 strips x 8 wells) 1 unit rat beta-NGF Standards (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons; neurotrophic factor; cutaneous innervation; growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta. -
Cellular localization
Secreted. -
Alternative names
- Beta nerve growth factor
- Beta NGF
- HSAN5
see all -
Database links
- Entrez Gene: 310738 Rat
- SwissProt: P25427 Rat
- Unigene: 22168 Rat
Images
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Typical Standard Curve
Representative standard curve using ab193736
