Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GALT antibody [EPR12555] (ab178406)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12555] to GALT
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-GALT antibody [EPR12555]
    See all GALT primary antibodies

  • Description

    Rabbit monoclonal [EPR12555] to GALT

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human GALT aa 50-150. The exact sequence is proprietary.
    Database link: P07902

  • Positive control

    • WB: HeLa, A549, K562, MDA-MB-435 and HepG2 whole cell lysate (ab7900); Human fetal brain and fetal liver lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GALT antibody [EPR12555] (ab178406)
    Western blot - Anti-GALT antibody [EPR12555] (ab178406)
    All lanes : Anti-GALT antibody [EPR12555] (ab178406) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : GALT knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 43 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab178406 observed at 43 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab178406 Anti-GALT antibody [EPR12555] was shown to specifically react with Galactose-1-Phosphate Uridylyltransferase in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265027 (knockout cell lysate ab257958) was used. Wild-type and Galactose-1-Phosphate Uridylyltransferase knockout samples were subjected to SDS-PAGE. ab178406 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-GALT antibody [EPR12555] (ab178406)
    Western blot - Anti-GALT antibody [EPR12555] (ab178406)
    Lanes 1-2 : Anti-GALT antibody [EPR12555] (ab178406) at 1/1000 dilution
    Lanes 3-4 : Anti-GAPDH antibody [6C5] - Loading Control (ab8245) at 1/2000 dilution

    Lanes 1 & 3 : GALT knockout HAP1 cell lysate
    Lanes 2 & 4 : Wild-type HAP1 cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 43 kDa



     

    Lanes 1 and 2: Green signal from target – ab178406 observed at 43 kDa

    Lanes 3 and 4: Red signal from loading control – ab8245 observed at 37 kDa

    Lanes 5 and 6: Merged (red and green) signal

    ab178406 was shown to react with GALT when GALT knockout samples were used, along with additional cross-reactive bands. Wild-type and GALT knockout samples were subjected to SDS-PAGE. ab178406 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW)preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-GALT antibody [EPR12555] (ab178406)
    Western blot - Anti-GALT antibody [EPR12555] (ab178406)
    All lanes : Anti-GALT antibody [EPR12555] (ab178406) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : MDA-MB-435 cell lysate
    Lane 3 : Human fetal brain lysate
    Lane 4 : Human fetal liver lysate
    Lane 5 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 43 kDa

  • Immunoprecipitation - Anti-GALT antibody [EPR12555] (ab178406)
    Immunoprecipitation - Anti-GALT antibody [EPR12555] (ab178406)

    Western blot analysis on Immunoprecipitation pellet from either 1) Human fetal liver lysate, or 2) 1xPBS (negative control); showing GALT, immunoprecipitated using ab178406 at 1/10 dilution with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-GALT antibody [EPR12555] (ab178406)
    Anti-GALT antibody [EPR12555] (ab178406)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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