HRP Anti-PGC1 beta antibody [EPR12370] (ab199228)
![HRP Anti-PGC1 beta antibody [EPR12370] (ab199228)](https://www.abcam.com/ps/products/199/ab199228/Images/ab199228-246566-anti-pgc1-beta-antibody-epr12370-hrp-western-blot.jpg "HRP Anti-PGC1 beta antibody [EPR12370] (ab199228)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR12370] to PGC1 beta
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-PGC1 beta antibody [EPR12370]
See all PGC1 beta primary antibodies -
Description
HRP Rabbit monoclonal [EPR12370] to PGC1 beta -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and MCF-7 whole cell lysates and human fetal brain and human fetal heart lysates.
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General notes
This product may be covered by one or more of the following U.S. Patents.
6,166,192
6,426,411
6,525,178
6,872,391
6,908,987
7,250,283
7,091,006
7,354,738
8,105,774
8,445,233This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12370 -
Isotype
IgG -
Research areas
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Images
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Western blot - HRP Anti-PGC1 beta antibody [EPR12370] (ab199228)All lanes : HRP Anti-PGC1 beta antibody [EPR12370] (ab199228) at 1/5000 dilution
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : Brain (Human) Tissue Lysate - fetal normal tissue
Lane 4 : Heart (Human) Tissue Lysate - fetal normal tissue
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 113 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199228 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-PGC1 beta antibody [EPR12370] (ab199228)](https://www.abcam.com/ps/products/199/ab199228/Images/ab199228-2-benefits-of-recombinant-antibodies.png)
HRP Anti-PGC1 beta antibody [EPR12370] (ab199228)
