KAT2B/pCAF Inhibitor Screening Assay Kit (Fluorometric) (ab196991)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Inhibitor compounds
Overview
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Product name
KAT2B/pCAF Inhibitor Screening Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Inhibitor compounds -
Assay type
Enzyme activity -
Product overview
KAT2B/pCAF Inhibitor Screening Assay Kit (Fluorometric) (ab196991) is an assay where histone H3 peptide and Acetyl CoA are used as acetylation substrates. KAT2B/pCAF acetylates the Histone H3 peptide and generates Coenzyme A with a free thiol group (CoA-SH). CoA-SH reacts subsequently with a Thiol Detecting Probe in order to increase the amount of fluorescence in the solution, which can be measured at Ex/Em = 392/482 nm. In the presence of KAT2B/pCAF specific inhibitors, the enzymatic activity is reduced ot completely abolished resulting in decreased or total loss of fluorescence.
This assay kit is a simple, sensitive and rapid tool to screen potential pCAF inhibitors.
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Notes
KTA2B (K-(lysine) acetyltransferase 2B, EC 2.3.1.48), also known as P300/CBP-associated factor (pCAF), possesses intrinsic histone acetyltransferase (HAT) activity and is able to acetylated both histone H3 and histone H4. KTA2B/pCAF contributes to transcriptional activation by acetylating chromatin and transcription factors through its HAT activity. Deregulated HAT activity plays an important role in various cancers.
KTA2B/pCAF inhibitors have potential applications in cancer therapy.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Acetyl CoA 1 vial H3 Peptide 1 vial pCAF Assay Buffer 1 x 25ml pCAF Enzyme 1 x 40µl pCAF Inhibitor 1 x 10µl Thiol Detecting Probe 1 x 200µl -
Research areas
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Function
Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. -
Tissue specificity
Ubiquitously expressed but most abundant in heart and skeletal muscle. -
Sequence similarities
Belongs to the GCN5 family.
Contains 1 bromo domain.
Contains 1 N-acetyltransferase domain. -
Domain
The bromodomain mediates binding to HIV-1 Tat. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- CAF
- CREBBP associated factor
- GCN5
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Images
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KAT2B/pCAF Inhibitor Screening Assay Kit (Fluorometric)
Inhibition of KAT2B/pCAF activity by different concentrations of the KAT2B/pCAF Inhibitor (Garcinol).