Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (ab249232)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7278] to Hsp27 (phospho S82) - BSA and Azide free
  • Suitable for: Dot blot, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free
    See all Hsp27 primary antibodies

  • Description

    Rabbit monoclonal [EPR7278] to Hsp27 (phospho S82) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects Hsp27 phosphorylated at Serine 82.

  • Tested applications

    Suitable for: Dot blot, IP, WBmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249232 is the carrier-free version of ab155987. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249232 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (ab249232)
    Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (ab249232)
    All lanes : Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) with 44°C heat shock whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

    Predicted band size: 23 kDa
    Observed band size: 27 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This data was developed using ab155987, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (ab249232)
    Dot Blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (ab249232)

    This data was developed using ab155987, the same antibody clone in a different buffer formulation.

    Dot blot analysis of Lane 1: Hsp27 (pS82) phospho peptide and Lane 2: Hsp27 non-phospho peptide, labeling Hsp27 (phospho S82) with ab155987 at 1/1000 dilution. 5% NFDM/TBST was used as the blocking and diluting buffer. ab97051, a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody was used at 1/100000 dilution. Exposure time: 10 seconds.
  • Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (ab249232)
    Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (ab249232)
    All lanes : Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1500 dilution (purified)

    Lane 1 : Mouse heart
    Lane 2 : Rat heart

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 27 kDa why is the actual band size different from the predicted?



    This data was developed using ab155987, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (ab249232)
    Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (ab249232)
    Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1500 dilution (purified) + HeLa cell lysate treated with heat shock (44°C) at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 27 kDa why is the actual band size different from the predicted?



    This data was developed using ab155987, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (ab249232)
    Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (ab249232)

    This data was developed using ab155987, the same antibody clone in a different buffer formulation.

    ab155987 (purified) at 1/75 immunoprecipitating Hsp27 (phospho S82) in HeLa cell lysate treated with heat shock (44°C) (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.
  • Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (ab249232)
    Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (ab249232)
    All lanes : Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/200 dilution (unpurified)

    Lane 1 : Mouse heart
    Lane 2 : Rat heart

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 27 kDa why is the actual band size different from the predicted?



    This data was developed using ab155987, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (ab249232)
    Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (ab249232)
    Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/200 dilution (unpurified) + HeLa cells treated with heat shock (44°C) at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 27 kDa why is the actual band size different from the predicted?



    This data was developed using ab155987, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (ab249232)
    Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (ab249232)
    All lanes : Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1000 dilution (unpurified)

    Lane 1 : Untreated HeLa cell lysate
    Lane 2 : HeLa cell lysate treated with heat shock (44°C).

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 23 kDa



    This data was developed using ab155987, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (ab249232)
    Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (ab249232)

    This data was developed using ab155987, the same antibody clone in a different buffer formulation.

    ab155987 (unpurified) at 1/75 immunoprecipitating Hsp27 (phospho S82) in HeLa cell lysate treated with heat shock (44°C) (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.
  • Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (ab249232)
    Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (ab249232)

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