Anti-STIP1/STI1 antibody [EPR6606] (ab126753)
![Anti-STIP1/STI1 antibody [EPR6606] (ab126753)](https://www.abcam.com/ps/products/126/ab126753/Images/ab126753-140764-anti-stip1-antibody-epr6606-western-blot.jpg "Anti-STIP1/STI1 antibody [EPR6606] (ab126753)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6606] to STIP1/STI1
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-STIP1/STI1 antibody [EPR6606]
See all STIP1/STI1 primary antibodies -
Description
Rabbit monoclonal [EPR6606] to STIP1/STI1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB Human
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Immunogen
Synthetic peptide within Human STIP1/STI1 aa 450-550. The exact sequence is proprietary.
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Positive control
- Human ovarian carcinoma tissue; Jurkat, HeLa, BxPC3, and HepG2 whole cell lysate (ab7900).
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General notes
This product was previously labelled as STIP1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6606 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-STIP1/STI1 antibody [EPR6606] (ab126753)All lanes : Anti-STIP1/STI1 antibody [EPR6606] (ab126753) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : BxPC3 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 63 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6606] (ab126753)](https://www.abcam.com/ps/products/126/ab126753/Images/ab126753-140763-anti-stip1-antibody-epr6606-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6606] (ab126753)ab126753, at 1/50 dilution, staining STIP1/STI1 in paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-STIP1/STI1 antibody [EPR6606] (ab126753)](https://www.abcam.com/ps/products/126/ab126753/Images/ab126753-336884-anti-stip1-antibody-epr6606-immunoprecipitation.jpg)
Immunoprecipitation - Anti-STIP1/STI1 antibody [EPR6606] (ab126753)ab126753 immunoprecipitating STIP1/STI1 in Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at a dilution of 1/30.
Blocking and diution buffer: 5% NFDM/TBST.
All lanes : Anti-STIP1/STI1 antibody [EPR6606] (ab126753) at 1/1000 dilution
Lane 1 : IP using ab126753 in Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
Observed band size: 63 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
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![Anti-STIP1/STI1 antibody [EPR6606] (ab126753)](https://www.abcam.com/ps/products/126/ab126753/Images/ab126753-4-benefits-of-recombinant-antibodies.png)
Anti-STIP1/STI1 antibody [EPR6606] (ab126753)
