Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MTCO1 antibody [EPR19628] - BSA and Azide free (ab213648)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19628] to MTCO1 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MTCO1 antibody [EPR19628] - BSA and Azide free
    See all MTCO1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19628] to MTCO1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, C6 and RAW 264.7 whole cell lysates; HeLa, Neuro-2a, C6 and SH-SY5Y mitochondria lysates; Mouse liver whole cell lysate; Mouse liver mitochondria lysate; Human fetal liver whole cell lysate. IHC-P: Human cardiac muscle and thyroid cancer tissues; Mouse and rat cardiac muscle tissues. ICC/IF: HeLa and Neuro-2a cells. Flow Cyt: HeLa cells.

  • General notes

    Ab213648 is the carrier-free version of ab203912. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab213648 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [EPR19628] - BSA and Azide free (ab213648)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [EPR19628] - BSA and Azide free (ab213648)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling MTCO1 with ab203912 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on Human cardiac muscle is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203912).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [EPR19628] - BSA and Azide free (ab213648)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [EPR19628] - BSA and Azide free (ab213648)

    Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling MTCO1 with ab203912 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on Human thyroid cancer is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203912).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [EPR19628] - BSA and Azide free (ab213648)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [EPR19628] - BSA and Azide free (ab213648)

    Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling MTCO1 with ab203912 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on mouse cardiac muscle is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203912).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [EPR19628] - BSA and Azide free (ab213648)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [EPR19628] - BSA and Azide free (ab213648)

    Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling MTCO1 with ab203912 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on rat cardiac muscle is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203912).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [EPR19628] - BSA and Azide free (ab213648)
    Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [EPR19628] - BSA and Azide free (ab213648)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling MTCO1 with ab203912 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on HeLa cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab203912 at 1/250 dilution followed by ab150120  at 1/1000 dilution.

    -ve control 2: ab7291 at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203912).

  • Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [EPR19628] - BSA and Azide free (ab213648)
    Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [EPR19628] - BSA and Azide free (ab213648)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling MTCO1 with ab203912 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on HeLa cell line.

    The nuclear counterstain is DAPI (blue).

    COX IV is detected with ab33985 (anti-COX IV mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab203912 at 1/250 dilution followed by ab150120  at 1/1000 dilution.

    -ve control 2: ab33985  at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203912).

  • Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [EPR19628] - BSA and Azide free (ab213648)
    Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [EPR19628] - BSA and Azide free (ab213648)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cell line) cells labeling MTCO1 with ab203912 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on Neuro-2a cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab203912 at 1/250 dilution followed by ab150120  at 1/1000 dilution.

    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203912).

  • Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [EPR19628] - BSA and Azide free (ab213648)
    Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [EPR19628] - BSA and Azide free (ab213648)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cell line) cells labeling MTCO1 with ab203912 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on Neuro-2a cell line.

    The nuclear counterstain is DAPI (blue).

    COX IV is detected with ab33985 (anti-COX IV mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab203912 at 1/250 dilution followed by ab150120  at 1/1000 dilution.

    -ve control 2: ab33985 at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203912).

  • Flow Cytometry - Anti-MTCO1 antibody [EPR19628] - BSA and Azide free (ab213648)
    Flow Cytometry - Anti-MTCO1 antibody [EPR19628] - BSA and Azide free (ab213648)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling MTCO1 with ab203912 at 1/70 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

    Cells were permeabilised with 90% methanol (diluted with 1X PBS).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203912).

  • Anti-MTCO1 antibody [EPR19628] - BSA and Azide free (ab213648)
    Anti-MTCO1 antibody [EPR19628] - BSA and Azide free (ab213648)

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