Astana Biomed Group, an authorized Abcam distributor in Central Asia

Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (ab154477)

Key features and details

  • Alexa Fluor® 488 Mouse monoclonal [1D6E1A8] to MTCO1
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Isotype: IgG2a

Overview

  • Product name

    Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8]
    See all MTCO1 primary antibodies

  • Description

    Alexa Fluor® 488 Mouse monoclonal [1D6E1A8] to MTCO1

  • Host species

    Mouse

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Caenorhabditis elegans, Zebrafish

  • Immunogen

    Full length protein corresponding to Human MTCO1.

  • Positive control

    • IF/ICC: HeLa and HDFn cells. Flow Cyt: HeLa cells.

  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

    ab154477 was previously used as a component in the MitoBiogenesis™ ICC Kit. The protocol for this kit is available here.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (ab154477)
    Flow Cytometry - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (ab154477)
    Overlay histogram showing HeLa cells stained with ab154477 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab154477, 1/100 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was mouse IgG2a Alexa Fluor® 488 (ab171464) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (ab154477)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (ab154477)

    ab154477 staining MTCO1 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab154477 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (pseudocolored in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (ab154477)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (ab154477)
    Immunocytochemistry with HeLa cells (100x) were stained with anti-MTCO1 Alexa-488 antibody (1.0 µg/mL, ab154477) in green and DAPI in blue, as a nuclear stain.
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (ab154477)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (ab154477)
    Immunocytochemistry with HDFn (100x) cells were stained with Anti-MTOC1 Alexa-488 antibody (1.0 µg/mL, ab154477) in green, Anti-HSP60 (1/1000, ab46798) as red, and DAPI in blue, as a nuclear stain. Secondary antibody used was goat anti-rabbit dyelight-594 (1/1000, ab96897).
  • Flow Cytometry - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (ab154477)
    Flow Cytometry - Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (ab154477)

    Overlay histogram showing HeLa cells stained with ab154477 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab154477, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

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