Immunohistochemical staining of paraffin embedded human kidney with purified ab181373 at a working dilution of 1 in 300. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181373).

Immunohistochemical analysis of paraffin-embedded human kidney clear cell carcinoma tissue labeling Klotho with unpurified ab181373 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181373).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Klotho with unpurified ab181373 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181373).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

The blots were produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane were blocked for an hour before being incubated with ab181373  (rabbit anti-Klotho antibody; diluted 5 ug/ml) for 24 hours at 4°C. Antibody binding was detected using infrared labelled goat anti-rabbit (green) antibody at 1:10,000 dilutions for 1 hour at room temperature before imaging.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181373).