Anti-PAM/Peptidyl-glycine alpha-amidating monooxygenase antibody (ab237488)
Key features and details
- Rabbit polyclonal to PAM/Peptidyl-glycine alpha-amidating monooxygenase
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PAM/Peptidyl-glycine alpha-amidating monooxygenase antibody -
Description
Rabbit polyclonal to PAM/Peptidyl-glycine alpha-amidating monooxygenase -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human PAM/Peptidyl-glycine alpha-amidating monooxygenase aa 338-497.
Database link: P19021 -
Positive control
- WB: HeLa whole cell lysate. IHC-P: Human endometrial cancer and heart tissues. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity greater than 95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PAM/Peptidyl-glycine alpha-amidating monooxygenase antibody (ab237488)Anti-PAM/Peptidyl-glycine alpha-amidating monooxygenase antibody (ab237488) at 1/500 dilution + HeLa whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 108 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAM/Peptidyl-glycine alpha-amidating monooxygenase antibody (ab237488)Paraffin-embedded human heart tissue stained for PAM/Peptidyl-glycine alpha-amidating monooxygenase using ab237488 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAM/Peptidyl-glycine alpha-amidating monooxygenase antibody (ab237488)Paraffin-embedded human endometrial cancer tissue stained for PAM/Peptidyl-glycine alpha-amidating monooxygenase using ab237488 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunocytochemistry/ Immunofluorescence - Anti-PAM/Peptidyl-glycine alpha-amidating monooxygenase antibody (ab237488)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PAM/Peptidyl-glycine alpha-amidating monooxygenase (green) using ab237488 at 1/66 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The nuclear counterstain was DAPI.