Anti-Klotho antibody (ab203576)
Key features and details
- Rabbit polyclonal to Klotho
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Klotho antibody
See all Klotho primary antibodies -
Description
Rabbit polyclonal to Klotho -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Klotho aa 400-500 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9UEF7 -
Positive control
- Mouse kidney tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.01% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody (ab203576)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse kidney tissue labeling Klotho with ab203576 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
-
Western blot - Anti-Klotho antibody (ab203576)Anti-Klotho antibody (ab203576) at 1/100 dilution + 293T cell lysate at 30 µg
Secondary
IRDye800CW Goat Anti-Rabbit IgG at 1/200000 dilution
Predicted band size: 116 kDa
Additional bands at: 148 kDa (possible glycosylated form)Klotho has several glycosylation sites, it looks like putative 116 kDa and the glycosylated form around 135 kDa are both correct.
-
Western blot - Anti-Klotho antibody (ab203576)Anti-Klotho antibody (ab203576) at 1/10000 dilution + Mouse kidney lysates at 1/300 dilution
Secondary
conjugated secondary antibody at 1/10000 dilution
Predicted band size: 116 kDa
