Anti-Mre11 antibody - C-terminal (ab227452)
Key features and details
- Rabbit polyclonal to Mre11 - C-terminal
- Suitable for: ICC/IF, IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Mre11 antibody - C-terminal
See all Mre11 primary antibodies -
Description
Rabbit polyclonal to Mre11 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment within Human Mre11 (C terminal). The exact sequence is proprietary.
Database link: P49959 -
Positive control
- WB: HeLa whole cell extract; IMR32 whole cell lysate. ICC/IF: SK-N-SH cells. IP: HeLa whole cell extract.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Mre11 antibody - C-terminal (ab227452)All lanes : Anti-Mre11 antibody - C-terminal (ab227452) at 1/1000 dilution
Lane 1 : Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : Mre11 shRNA transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 81 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-Mre11 antibody - C-terminal (ab227452)Anti-Mre11 antibody - C-terminal (ab227452) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg
Predicted band size: 81 kDa7.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody - C-terminal (ab227452)4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells stained for Mre11 (green) using ab227452 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
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Immunoprecipitation - Anti-Mre11 antibody - C-terminal (ab227452)Mre11 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 µg ab227452. Western blot was performed from the immunoprecipitate using ab227452. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HeLa whole cell extract.
Lane 2: Control IgG IP in HeLa whole cell extract.
Lane 3: ab227452 IP in HeLa whole cell extract.
