Anti-Mre11 antibody - ChIP Grade (ab12159)
Key features and details
- Rabbit polyclonal to Mre11 - ChIP Grade
- Suitable for: ChIP
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-Mre11 antibody - ChIP Grade
See all Mre11 primary antibodies -
Description
Rabbit polyclonal to Mre11 - ChIP Grade -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ChIP Human
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Immunogen
Synthetic peptide:
C-SQAESSKPTSKPK
conjugated to KLH, corresponding to amino acids 578-592 of S. cerevisiae Mre11.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-Mre11 antibody - ChIP Grade (ab12159)
A Saccharomyces cerevisiae strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes). After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin.
Chromatin was immunoprecipitated with the antibody at the stated dilutions. Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies. The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus). PCR products were displayed on a polyacrylamide gel, stained with SyBR Green (Invitrogen), and detected using a Fuji scanning fluorometer.
