Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Mre11 antibody [12D7] (ab214)

Key features and details

  • Mouse monoclonal [12D7] to Mre11
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Reacts with: Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Mre11 antibody [12D7]
    See all Mre11 primary antibodies

  • Description

    Mouse monoclonal [12D7] to Mre11

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Amino acids 182-582 of Mre11 expressed in E. coli.

  • Positive control

    • WB HEK-293T, A431, HeLa, HepG2, PC-12 whole cell lysate; ICC: HeLa cells.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 10th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Images

  • Western blot - Anti-Mre11 antibody [12D7] (ab214)
    Western blot - Anti-Mre11 antibody [12D7] (ab214)
    All lanes : Anti-Mre11 antibody [12D7] (ab214) at 1/1000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : anti-mouse IgG HRP-conjugated antibody

    Predicted band size: 79 kDa



    7.5% SDS-PAGE

  • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)
    Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)

    Immunocytochemical analysis of, 4% paraformaldehyde-fixed at RT for 15 min, HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Mre-11 (green) with ab214 at 1/200 dilution. Blue: Hoechst 33342 staining. Scale bar= 10 μm.

  • Flow Cytometry - Anti-Mre11 antibody [12D7] (ab214)
    Flow Cytometry - Anti-Mre11 antibody [12D7] (ab214)

    Overlay histogram showing HeLa cells stained with ab214 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab214, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Mre11 antibody [12D7] (ab214)
    Western blot - Anti-Mre11 antibody [12D7] (ab214)
    Anti-Mre11 antibody [12D7] (ab214) at 1/500 dilution + PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg

    Secondary
    Anti-mouse IgG HRP-conjugated antibody

    Developed using the ECL technique.

    Predicted band size: 79 kDa



    7.5% SDS-PAGE

    The signal was developed with Trident ECL plus-Enhanced.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Mre11 antibody [12D7] (ab214)
    Western blot - Anti-Mre11 antibody [12D7] (ab214)
    All lanes : Anti-Mre11 antibody [12D7] (ab214) at 1/1000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : Human Mre-11-transfected HEK-293T whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : anti-mouse IgG HRP-conjugated antibody

    Predicted band size: 79 kDa



    This image was generated using the ascites version of the product.

    7.5% SDS-PAGE

  • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)
    Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214) Image supplied by Dr Domenico Delia, Istituto Nazionale Tumori, Italy.

    Indirect immunofluorescence to detect localisation of Mre11 in normal lymphoblastoid cells.
    (Panel D - negative control).

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Mre11 antibody [12D7] (ab214)
    Western blot - Anti-Mre11 antibody [12D7] (ab214) This image is courtesy of an anonymous Abreview
    All lanes : Anti-Mre11 antibody [12D7] (ab214) at 1/500 dilution

    Lane 1 : Mouse breast cancer cell line - whole cell lysate. Transfected with vector control.
    Lane 2 : Mouse breast cancer cell line - whole cell lysate. Transfected with knockdown shRNA targeting Mre11 gene.

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated Goat anti-mouse IgG polyclonal at 1/1000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 79 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This image was generated using the ascites version of the product.

    See Abreview

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