Recombinant human BTK (mutated C481S) protein (ab204166)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% Densitometry
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: WB, SDS-PAGE, Functional Studies
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Product name
Recombinant human BTK (mutated C481S) protein
See all BTK proteins and peptides -
Biological activity
The specific activity of ab204166 was determined to be 70 nmol/min/mg.
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Purity
> 95 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MAAVILESIFLKRSQQKKKTSPLNFKKRLFLLTVHKLSYYEYDFERGRRG SKKGSIDVEKITCVETVVPEKNPPPERQIPRRGEESSEMEQISIIERFPY PFQVVYDEGPLYVFSPTEELRKRWIHQLKNVIRYNSDLVQKYHPCFWIDG QYLCCSQTAKNAMGCQILENRNGSLKPGSSHRKTKKPLPPTPEEDQILKK PLPPEPAAAPVSTSELKKVVALYDYMPMNANDLQLRKGDEY FILEESN LPWWRARDKNGQEGYIPSNYVTEAEDSIEMYEWYSKHMTRSQAEQLLKQE GKEGGFIVRDSSKAGKYTVSVFAKSTGDPQGVIRHYVVCSTPQSQYYLAE KHLFSTIPELINYHQHNSAGLISRLKYPVSQQNKNAPSTAGLGYGSWEID PKDLTFLKELGTGQFGVVKYGKWRGQYDVAIKMIKEGSMSEDEFIEEAKV MMNLSHEKLVQLYGVCTKQRPIFIITEYMANGSLLNYLREMRHRFQTQQL LEMCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVSDFGLSRYVLD DEYTSSVGSKFPVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPY ERFTNSETAEHIAQGLRLYRPHLASEKVYTIMYSCWHEKADERPTFKILL SNILDVMDEES -
Predicted molecular weight
75 kDa including tags -
Amino acids
1 to 659 -
Modifications
mutated C481 -
Tags
His tag N-Terminus -
Additional sequence information
NM_000061
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.82% Sodium phosphate, 1.74% Sodium chloride, 0.002% PMSF, 0.004% DTT, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human BTK (mutated C481S) protein (ab204166)The specific activity of BTK (ab204166) was determined to be 65 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human BTK (mutated C481S) protein (ab204166)SDS PAGE analysis of ab204166 -
SDS-PAGE - Recombinant human BTK (mutated C481S) protein (ab204166)SDS PAGE analysis of ab204166 -
SDS-PAGE - Recombinant human BTK (mutated C481S) protein (ab204166)Analysis of the purity of ab204166 by densitometry.
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Functional Studies - Recombinant human BTK (mutated C481S) protein (ab204166)Kinase assay demonstrating specific activity of ab204166.
