Recombinant human BTK protein (ab205800)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
-
Product name
Recombinant human BTK protein
See all BTK proteins and peptides -
Biological activity
The specific activity of ab205800 was determined to be 43 nmol/min/mg.
-
Purity
> 90 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MAAVILESIFLKRSQQKKKTSPLNFKKRLFLLTVHKLSYYEYDFERGRRG SKKGSIDVEKITCVETVVPEKNPPPERQIPRRGEESSEMEQISIIERFPY PFQVVYDEGPLYVFSPTEELRKRWIHQLKNVIRYNSDLVQKYHPCFWIDG QYLCCSQTAKNAMGCQILENRNGSLKPGSSHRKTKKPLPPTPEEDQILKK PLPPEPAAAPVSTSELKKVVALYDYMPMNANDLQLRKGDEYFILEESNLP WWRARDKNGQEGYIPSNYVTEAEDSIEMYEWYSKHMTRSQAEQLLKQEGK EGGFIVRDSSKAGKYTVSVFAKSTGDPQGVIRHYVVCSTPQSQYYLAEKH LFSTIPELINYHQHNSAGLISRLKYPVSQQNKNAPSTAGLGYGSWEIDPK DLTFLKELGTGQFGVVKYGKWRGQYDVAIKMIKEGSMSEDEFIEEAKVMM NLSHEKLVQLYGVCTKQRPIFIITEYMANGCLLNYLREMRHRFQTQQLLE MCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVSDFGLSRYVLDDE YTSSVGSKFPVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYER FTNSETAEHIAQGLRLYRPHLASEKVYTIMYSCWHEKADERPTFKILLSN ILDVMDEES -
Predicted molecular weight
78 kDa including tags -
Amino acids
1 to 659 -
Tags
His tag N-Terminus
-
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.82% Sodium phosphate, 1.74% Sodium chloride, 25% Glycerol (glycerin, glycerine), 0.002% PMSF, 0.004% DTTThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
-
Functional Studies - Recombinant human BTK protein (ab205800)The specific activity of BTK (ab205800) was determined to be 35 nmol/min/mg as per activity assay protocol and was equivalent to 49 nmol/min/mg as per radiometric assay
-
SDS-PAGE - Recombinant human BTK protein (ab205800)SDS PAGE analysis of ab205800 -
SDS-PAGE - Recombinant human BTK protein (ab205800)SDS PAGE analysis of ab205800 -
Functional Studies - Recombinant human BTK protein (ab205800)Kinase Assay demonstrating specific activity of ab205800
-
SDS-PAGE - Recombinant human BTK protein (ab205800)SDS-PAGE analysis of ab205800.
