Human IgG3 ELISA Kit (ab137981)
Key features and details
- Sensitivity: 0.22 ng/ml
- Range: 0.391 ng/ml - 25 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IgG3 ELISA Kit
See all IgG3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 3.3% Inter-assay Sample n Mean SD CV% Overall 9.2% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
> 0.22 ng/ml -
Range
0.391 ng/ml - 25 ng/ml -
Recovery
102 %
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Assay time
4h 0m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s IgG3 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IgG3 in plasma, serum, urine, saliva, milk, and cell culture supernatants.
An IgG3 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IgG3 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IgG3 captured in plate.
Get results in 90 minutes with Human IgG3 ELISA Kit (ab201284) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human IgG3 Antibody 1 x 120µl Chromogen Substrate 1 x 8ml IgG3 Microplate (12 x 8 well strips) 1 unit IgG3 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Relevance
IgG3, like IgG1, activates complement and has an affinity for fc receptors; thus IgG1 and IgG3 are the more effective of the IgG subclasses. An IgG3 deficiency is associated with Wiscott-Aldrich disease, systemic lupus erythematosus, juvenile diabetes mellitus, and peridontal infections. IgG anti-viral antibodies are restricted to IgG subclasses 1 and 3, with IgG3 antibodies being the first to appear in response to infection. -
Alternative names
- DKFZp686H11213
- FLJ39988
- FLJ40036
see all -
Database links
- Entrez Gene: 3502 Human
- Omim: 147120 Human
- SwissProt: P01860 Human
- Unigene: 510635 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab137981
